不同潮气量机械通气对大鼠肺Toll样受体4及其相关信号链酶表达的影响

发布时间：2018-02-15 14:23

本文关键词： Toll样受体肿瘤坏死因子受体相关因子白细胞介素受体相关激酶核转录因子-κB　出处：《广东医学》2014年11期 　论文类型：期刊论文

【摘要】：目的探讨Toll样受体4(TLR4)及其相关信号链酶在不同潮气量机械通气大鼠肺组织中的表达变化。方法按随机数字表法将40只SD大鼠分为5组,每组8只。A组仅切开气管保留自主呼吸,B1组和B2组以潮气量6 mL/kg分别通气1 h和2 h,C1组和C2组以潮气量30 mL/kg分别通气1 h和2 h。A组在气管切开即刻,其余各组在机械通气结束时处死大鼠。光镜下观察各组大鼠肺组织病理改变,免疫组化法检测肺组织中TLR4、肿瘤坏死因子受体相关因子6(TRAF6)、白细胞介素1受体相关激酶4(IRAK4)及核转录因子-κB(NF-κB)p65蛋白的表达,RT-PCR检测TLR4 mRNA的表达,测定肺组织髓过氧化物酶(MPO)的活性,计算肺湿干重比值(W/D比值),ELISA法测定支气管肺泡灌洗液中肿瘤坏死因子-α(TNF-α)的浓度。结果 A组、B1组、B2组之间肺组织TLR4 mRNA表达,TLR4、TRAF6、IRAK4、NF-κBp65蛋白的表达,W/D比值,MPO活性和TNF-α浓度差异均无统计学意义(P0.05)。与相应B1、B2组比较,C1、C2组TLR4 mRNA和TLR4、TRAF6、IRAK4、NF-κBp65蛋白表达明显上调,W/D比值、MPO活性和TNF-α浓度明显升高,差异均有统计学意义(P0.05)。随通气时间延长,C2组各指标较C1组改变更为明显(P0.05)。结论 TLR4及其相关信号链酶在肺组织中的表达参与了机械通气相关性肺损伤的发生与发展。
[Abstract]:Objective to investigate the expression of Toll like receptor 4 (TLR4) and its signal chain enzymes in lung tissue of rats with different tidal volume mechanical ventilation. Methods 40 SD rats were randomly divided into 5 groups. 8 rats in each group were ventilated with tidal volume for 1 h and 2 mL/kg for group B1 and group B2 for 1 h and 2 hours respectively with tidal volume for 30 mL/kg and 1 h and 2 h after tracheotomy in group B 1 and group B 2, respectively. The other groups were killed at the end of mechanical ventilation. The pathological changes of lung tissue in each group were observed under light microscope. The expression of TLR4, TNF- receptor related factor (TLR4), interleukin-1 receptor-associated kinase (IL-1RAK4), nuclear transcription factor- 魏 B, NF- 魏 B p65 and myeloperoxidase (MPO) activity in lung tissue were detected by RT-PCR. The concentration of tumor necrosis factor- 伪 (TNF- 伪) in bronchoalveolar lavage fluid (BALF) was determined by W- / D ratio Elisa. Results the expression of TLR4 mRNA in lung tissue between group A and B _ 1 group B _ 2 was determined. The expression of TLR4 mRNA and TLR4TRAF6TRAF6 / IRAK4NF- 魏 Bp65 protein in C _ 1 / C _ 2 group was significantly up-regulated, and the activity of TLR4 and the concentration of TNF- 伪 were significantly increased compared with the corresponding B1C _ 2 group. The difference was statistically significant (P 0.05). The changes of the indexes in C 2 group were more obvious than those in C 1 group. Conclusion the expression of TLR4 and its related signal chain enzymes in lung tissue is involved in the occurrence and development of mechanical ventilation associated lung injury.
【作者单位】：广州军区广州总医院急诊科;桂林医学院附属医院重症科;
【分类号】：R563.8

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