胆红素对急性肺损伤大鼠水通道蛋白1、5表达的影响

发布时间：2018-02-24 02:27

本文关键词： 急性肺损伤胆红素水通道蛋白1 水通道蛋白5 大鼠肺水肿　出处：《山西医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：探讨胆红素对脂多糖所致急性肺损伤大鼠的水通道蛋白1、5是否存在调节作用,探讨其保护作用机制。方法：雄性wistar大鼠18只，随机分为生理盐水对照组(C组)，脂多糖模型组(L组)，胆红素预处理组(B组)，每组各6只。每组于造模后4h，处死大鼠。测定每组肺湿/干重比值， ELISA法测血清中IL-8含量，肺组织中cAMP含量，免疫组化法测定每组水通道蛋白1、5的表达变化，观察各组肺组织病理改变。结果：1、血清中IL-8的含量与C组比较L组IL-8含量明显增加，两组间差异有统计学意义（P0.05）。B组IL-8含量较L组减少，但仍高于C组，其与其他两组间比较均有统计学意义（P0.05）。2、肺组织中cAMP含量L组与C组比较cAMP含量明显降低，两组间差异有统计学意义（P0.05），B组cAMP含量较L组增多，但仍低于C组,其与其他两组间比较差异均有统计学意义（P0.05）。3、AQP-1，AQP-5表达的变化L组与C组比较AQP-1表达明显下降（P0.05），B组AQP-1表达明显上调，与C组之间无显著差异（P0.05）。L组较C组AQP-5表达明显下降（P0.05），B组AQP-5表达上升，但与C组之间差异无统计学意义（P0.05）。4、大鼠肺W/D值变化L组W/D比值明显高于C组，差异有统计学意义（P0.05）,B组较L组W/D比值降低，但仍高于C组（P0.05）。5、大鼠肺组织病理学改变C组无明显炎症变化，L组光镜下可见不同程度的肺泡结构破坏，肺泡间隔明显增宽，肺泡腔及间质内大量炎性细胞浸润，肺间质水肿，肺泡腔内有渗出液，局部出现肺不张，肺萎陷。B组肺水肿程度减轻，炎性细胞浸润减少，肺泡腔无明显渗出。结论：胆红素可能是通过调节水通道蛋白1、5表达改善水代谢，减轻肺水肿状态，，从而对急性肺损伤具有保护作用。
[Abstract]:Aim: to investigate the regulatory effect of bilirubin on aquaporin-1 5 in rats with acute lung injury induced by lipopolysaccharide (LPS) and its protective mechanism. Methods: eighteen male wistar rats were used. The rats were randomly divided into normal saline control group (n = 6), lipopolysaccharide model group (n = 6) and bilirubin pretreatment group (n = 6). The rats in each group were killed at 4 hours after modeling. The lung wet / dry weight ratio of each group was measured and the serum IL-8 content was measured by ELISA method. The content of cAMP and the expression of aquaporin 1 5 in each group were determined by immunohistochemical method. The pathological changes of lung tissue were observed in each group. Results compared with group C, the content of IL-8 in serum of group 1 was significantly higher than that of group C, and the content of IL-8 in group B was significantly lower than that in group L, but still higher than that in group C, and the content of IL-8 in group B was significantly lower than that in group C. Compared with the other two groups, the content of cAMP in lung tissue of group L and group C was significantly lower than that of group C, and the content of cAMP in group B was significantly higher than that in group L, but still lower than that in group C. There was a significant difference between the two groups in the expression of P0.05N. 3AQP-1AQP-5. The expression of AQP-1 in group L was significantly lower than that in group C. The expression of AQP-1 was up-regulated in group B (P 0.05). There was no significant difference between group C and group C in the expression of AQP-5, but there was no significant difference between group C and group C in the expression of AQP-5. The ratio of W / D in group L was significantly higher than that in group C. The ratio of W / D in group B was significantly lower than that in group L, but still higher than that in group C (P 0.05). The pathological changes of lung tissue in group C had no obvious inflammatory changes. In group L, there were different degrees of destruction of alveolar structure and widening of alveolar septum under light microscope. A large number of inflammatory cells were infiltrated in alveolar cavity and interstitial, pulmonary interstitial edema, exudate in alveolar cavity, atelectasis occurred locally, the degree of pulmonary edema in group B was reduced, the infiltration of inflammatory cells was decreased, and there was no obvious exudation in alveolar cavity. Conclusion: bilirubin may improve water metabolism and alleviate pulmonary edema by regulating the expression of aquaporin 1, which may have protective effect on acute lung injury.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.9

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