辛伐他汀对肺纤维化鼠肺组织血管新生及VEGF和PF4基因表达的影响

发布时间：2018-02-24 19:56

本文关键词： 肺纤维化血管新生辛伐他汀血管内皮生长因子血小板第四因子　出处：《实用医学杂志》2014年17期 　论文类型：期刊论文

【摘要】：目的:观察辛伐他汀对肺纤维化大鼠肺组织血管新生及部分调控基因表达的影响。方法:选取SD大鼠96只,随机分为4组:空白组(A组)、模型组(B组)、泼尼松组(C组)、辛伐他汀组(D组),建模后,于7、14、28 d随机处死8只,取肺组织为检测标本,消化法测定羟脯氨酸(HYP),免疫组化法测血管新生微血管密度(MVD)及血管内皮生长因子(VEGF)、血小板第四因子(PF4)蛋白的表达,RT-PCR法测VEGF及PF4mRNA表达。结果:(1)C、D组HYP较B组减轻(均P0.01);(2)B、C、D组MVD及VEGF表达高于A组,PF4表达低于A组(均P0.01);D组MVD及VEGF表达低于B组,PF4表达高于B组(均P0.05)。结论:辛伐他汀有改善纤维化作用,其机制可能与其调节肺组织内VEGF及PF4表达、抑制病理性血管新生有关。
[Abstract]:Objective: to observe the effect of simvastatin on angiogenesis and partially regulated gene expression in lung tissue of rats with pulmonary fibrosis. Methods: 96 SD rats were selected. They were randomly divided into four groups: control group A, model group B, prednisone group C, simvastatin group D group. After modeling, 8 rats were killed at random on day 7, 1428, and lung tissues were taken as test specimens. The expression of hydroxyproline (HYPX), vascular endothelial growth factor (VEGF), vascular endothelial growth factor (VEGF), hydroxyproline (Hyp), VEGF and PF4mRNA were measured by RT-PCR. Results the HYP in group C was lower than that in group B (all of the results were lower than those in group B) (P < 0.01). The expression of MVD and VEGF in group D was higher than that in group A (P 0.01). The expression of MVD and VEGF in group D was lower than that in group B (P 0.05). Conclusion: simvastatin can improve fibrosis. The mechanism may be related to its regulation of VEGF and PF4 expression and inhibition of pathological angiogenesis.
【作者单位】：南华大学附属第二医院呼吸内科;南华大学附属第二医院心血管内科;
【基金】：湖南省教育科研项目(编号:11C1106)
【分类号】：R563

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