可溶性B7-H2及IL-6在支气管哮喘急性发作期患者血清中的表达及其临床意义

发布时间：2018-02-25 20:24

本文关键词： 支气管哮喘 s B7-H2 IL-6　出处：《宁夏医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过测定可溶性B7-H2(s B7-H2)、白介素6(IL-6)在支气管哮喘急性发作期患者和健康体检者血清中的表达水平,并分析二者与临床常用指标呼出气一氧化氮(Fe NO)、血嗜酸性粒细胞计数(EOS)的相关性,评价其对哮喘诊断、急性发作期病情严重程度判别及气道炎症水平评估的临床价值。方法:收集自2015年1月-2016年1月甘肃省人民医院呼吸科住院部及门诊支气管哮喘急性发作期患者60例为哮喘组,其中轻度组15例,中重度组45例,同时收集体检中心健康体检者20例为对照组。采用ELISA法检测各组人群血清s B7-H2、IL-6表达水平,收集哮喘组EOS、Fe NO临床常用指标。分析s B7-H2、IL-6在哮喘急性发作期表达特点及其与临床常用指标的相关性,用SPSS21.0统计软件对数据进行分析,α=0.05为检验水准,P0.05表示差异有统计学意义。结果:1.哮喘组血清s B7-H2、IL-6表达水平较对照组明显升高,差异有统计学意义(P0.01,P0.01);哮喘中重度组血清s B7-H2、IL-6表达水平高于哮喘轻度组,差异有统计学意义(P0.01,P0.01)。2.哮喘组血清s B7-H2值与EOS(r=0.337,P0.05)、Fe NO(r=0.651,P0.01)均呈正相关;哮喘组血清IL-6值与EOS(r=0.286,P0.05)、Fe NO(r=0.764,P0.01)均呈正相关。结论:1.哮喘组血清s B7-H2及IL-6表达水平较对照组升高,哮喘中重度组血清s B7-H2及IL-6表达水平较轻度组升高,说明二者的测定对哮喘的诊断及病情严重程度评估有一定的参考价值。2.s B7-H2及IL-6与临床常用指标EOS、Fe NO存在正相关性,说明二者可作为哮喘气道炎症的评估指标。
[Abstract]:Objective: to determine the expression of soluble B7-H2OS-B7-H2P, interleukin-6 (IL-6) in the serum of patients with acute attack of bronchial asthma and healthy controls. The correlation between them and exhaled nitric oxide (no) and eosinophil count (EOS) was analyzed, and the diagnosis of asthma was evaluated. Methods: from January 2015 to January 2016, 60 cases of acute asthma patients in respiratory department and outpatient department of Gansu Provincial people's Hospital were selected as asthma group. There were 15 cases in mild group, 45 cases in moderate and severe group, and 20 cases as control group. The expression of sB7-H2O6 in serum of all groups was detected by ELISA method. To collect the common clinical indexes of EOS-Fe no in asthma group, to analyze the expression characteristics of sB7-H _ 2O _ 6 in acute attack of asthma and its correlation with the commonly used clinical indexes. The data were analyzed by SPSS21.0 software. The results showed that there was a significant difference between the two groups. Results the serum sB7-H2O6 expression level in asthma group was significantly higher than that in control group, and the level of serum sB7-H2O6 in asthma group was significantly higher than that in control group. The expression of sB7-H2O6 in the middle and severe asthmatic group was higher than that in the mild asthmatic group, and the difference was statistically significant. The serum sB7-H2 level in the asthmatic group was positively correlated with that in the EOS-0.337P0.05FeNOr0.651P0.01) group (P < 0.05). The serum sB7-H2 level in the asthmatic group was significantly higher than that in the mild asthmatic group (P < 0.01). The serum sB7-H2 level in the asthmatic group was significantly higher than that in the mild asthmatic group (P < 0.05). The serum IL-6 level of asthma group was positively correlated with that of EOSN 0.286 (P 0.05). Conclusion the serum sB7-H2 and IL-6 expression levels in asthmatic group were higher than those in control group, and the expression levels of sB7-H2 and IL-6 in asthmatic group were higher than those in mild asthmatic group, and the level of serum sB7-H2 and IL-6 in asthma group was higher than that in mild group, and the level of serum sB7-H2 and IL-6 in asthmatic group was higher than that in control group. It is suggested that the determination of both of them has certain reference value for the diagnosis of asthma and assessment of severity of asthma. 2.sB7-H2 and IL-6 have positive correlation with the common clinical index EOS-Fe no, which indicates that they can be used as an evaluation index of asthma airway inflammation.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R562.25

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