Th2细胞和相关细胞因子在继发性间质性肺炎中的表达及意义

发布时间：2018-02-26 08:11

本文关键词： 辅助性T细胞2 白介素-10 转化生长因子β1 继发性间质性肺炎肺纤维化　出处：《广西医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的观察皮下注射博来霉素致间质性肺炎小鼠模型中Th2细胞及相关细胞因子的变化,探讨博来霉素致继发性间质性肺炎的可能机制。方法将20只Balb/c小鼠随机分为2组：对照组(A组)：皮下注射磷酸盐缓冲液,10只；模型组(B组)：皮下注射博来霉素,10只。Masson和HE染色后观察小鼠肺部病理的改变,评估纤维化和炎症程度。流式细胞术检测外周血和肺组织Th2细胞的比例,免疫荧光定量PCR法检测肺组织TGF-β1mRNA和IL-10mRNA的表达量,ELISA法检测外周血LTGF-β1和IL-10的含量,并分析这些指标之间的相关性。结果(1)B组小鼠肺纤维化评分[3.5(3,5)]、肺部炎症评分[2(2,3)]均高于A组[0.5(0,1)和0(0,1)],(均P0.05)；(2)外周血和肺组织Th2细胞比例B组[(3.32±2.13)%和(5.72±3.37)%]明显高于A组[(1.21±0.63)%和(1.51±0.34)%]；(3)外周血TGF-β1含量B组[(1426.31±137.08)pg/ml]明显高于A组[(1138.44±92.61)pg/ml],外周血IL-10含量B组[(122.09±47.62) pg/ml]明显高于A组[(76.82±25.74)pg/ml](均P0.05)；(4)肺组织TGF-β1mRNA和IL-10mRNAB组[0.2100(0.0418,0.4700)和0.0261(0.0040,0.0402)]较A组[0.0169(0.0023,0.0426)和0.0003(0.0002,0.0043)]明显增高(均P0.05)；(5)外周血和肺部Th2细胞与纤维化评分、炎症评分呈正相关(r值分别为0.618、0.623和0.589、0.567,均P0.05),肺部TGF-β1mRNA和IL-10mRNA与肺部纤维化评分、炎症评分正相关(r值为0.782、0.620和0.516、0.592,均P0.05),肺部Th2细胞与肺部TGF-β1mRNA、IL-10mRNA、外周血TGF-β1、IL-10的含量正相关,均P0.05。结论皮下注射博来霉素小鼠肺部出现炎症和纤维化的病理改变,Th2细胞、TGF-β1、IL-10在博来霉素致继发性间质性肺炎小鼠模型中的表达是增高的；Th2细胞、TGF-β1、IL-10与肺纤维化正相关,提示博来霉素致继发性间质性肺炎的发病中存在以Th2细胞调节异常的免疫失衡。
[Abstract]:Objective to observe the changes of Th2 cells and related cytokines in murine model of interstitial pneumonia induced by bleomycin, and to explore the possible mechanism of bleomycin induced secondary interstitial pneumonia. Methods Twenty Balb/c mice were randomly divided into two groups: control group A (n = 10) and model group B (n = 10). The ratio of Th2 cells in peripheral blood and lung tissue was detected by flow cytometry, the expression of TGF- 尾 1 mRNA and IL-10mRNA in lung tissue was detected by immunofluorescence quantitative PCR assay, and the levels of LTGF- 尾 1 and IL-10 in peripheral blood were detected by Elisa. The correlation between these indexes was analyzed. Results the pulmonary fibrosis score and pulmonary inflammation score were significantly higher in group B than those in group A [0.5 and 0. 05%, respectively)] (P0. 05%). The percentage of Th2 cells in peripheral blood and lung tissue in group B [3. 32 卤2. 1313% and 5. 72 卤3. 37%] was significantly higher than that in group A [1. 21 卤0. 63% and 1. 51 卤0. 34%]. The level of TGF- 尾 1 in peripheral blood of group B [1 426.31 卤137. 08 PG / ml] was significantly higher than that in group A [1. 21 卤0. 63% and 1. 51 卤0. 34%]. In group A (1138.44 卤92.61g / ml), the level of IL-10 in peripheral blood of group B [122.09 卤47.62 pg/ml] was significantly higher than that in group A [76.82 卤25.74pg / ml] (all P0.05H4) and IL-10mRNAB group (0.21000.00.04184180.4700 and 0.0261n 0.00400.402) were significantly higher than those in group A [0.0169t 0.00230.0426 and 0.00030.0000.00020.0043] (all P 0.05) and pulmonary Th2 cells and fibrosis scores were significantly higher than those in group A (P 0.05). The positive correlation of inflammatory score was 0.618 ~ 0.623 and 0.589 ~ 0.567, respectively (P < 0.05). The positive correlation between lung TGF- 尾 _ 1 mRNA and IL-10mRNA and pulmonary fibrosis score was 0.7820.62 and 0.516 ~ 0.592respectively (P 0.05). The positive correlation between lung Th2 cells and lung TGF- 尾 _ 1mRNA-IL-10 mRNA and peripheral blood TGF- 尾 _ 1mRNA-IL-10 was all P 0.055.The positive correlation between TGF- 尾 _ 1mRNA and TGF- 尾 _ 1mRNA-IL-10 in lung was significant (P < 0.05), and the positive correlation between TGF- 尾 _ (1) mRNA and TGF- 尾 _ (1) IL-10 in peripheral blood was also found. Conclusion the pathological changes of pulmonary inflammation and fibrosis in bleomycin induced by subcutaneous injection of bleomycin in mice with pulmonary fibrosis may indicate that the expression of TGF- 尾 1 and IL-10 in Th2 cells is positively correlated with pulmonary fibrosis in the murine model of bleomycin induced secondary interstitial pneumonia, and the expression of TGF- 尾 1 and IL-10 is positively correlated with pulmonary fibrosis in murine models of secondary interstitial pneumonia induced by bleomycin. The results suggest that the immune imbalance of Th2 cells is abnormal in the pathogenesis of secondary interstitial pneumonia caused by bleomycin.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.1

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