HHIP基因多态性与新疆哈萨克族慢性阻塞性肺疾病易感性的关系研究

发布时间：2018-02-26 12:03

本文关键词： 肺疾病慢性阻塞性 HHIP基因多态性单核苷酸　出处：《中国全科医学》2017年19期 　论文类型：期刊论文

【摘要】：目的探讨HHIP基因rs13118928、rs1828591位点单核苷酸多态性(SNPs)与新疆哈萨克族慢性阻塞性肺疾病(COPD)易感性的关系。方法选取2014年10月—2016年5月在伊犁州友谊医院、新华医院、石河子大学医学院第一附属医院呼吸科住院及门诊就诊的COPD患者214例为病例组。另选取同期在这几家医院进行体检的健康者350例为对照组。统一使用美国Medgraphics公司的肺功能仪进行用力肺活量(FVC)、第1秒用力呼气末容积(FEV1)测定,并记录FEV1占预计值百分比、FEV1/FVC。所有受试者抽取外周静脉血标本,提取标本DNA,运用im LDRTM多重SNP分型技术检测HHIP基因各位点的多态性。结果两组rs13118928位点基因型及等位基因分布比较,差异均无统计学意义(P0.05)。两组rs1828591位点基因型及等位基因分布比较,差异均无统计学意义(P0.05)。病例组rs13118928位点不同基因型患者FEV1占预计值百分比、FEV1/FVC比较,差异均无统计学意义(P0.05)。病例组rs1828591位点不同基因型患者FEV1占预计值百分比、FEV1/FVC比较,差异均无统计学意义(P0.05)。两组HHIP基因rs13118928、rs1828591两位点单体型分布比较,差异无统计学意义(P0.05)。结论 HHIP基因rs13118928、rs1828591位点SNPs与新疆哈萨克族COPD易感性无明显关系。
[Abstract]:Objective to investigate the relationship between HHIP gene rs13118928rs1828591 single nucleotide polymorphism (SNPs) and susceptibility to chronic obstructive pulmonary disease (HHIP) in Xinjiang Kazak nationality. Methods from October 2014 to May 2016, Xinghua Hospital, Yili Zhou Friendship Hospital, was selected. 214 cases of COPD patients in respiratory department and outpatient clinic of the first affiliated Hospital of Shihezi University Medical College were selected as the case group, and 350 healthy persons who underwent physical examination in these hospitals during the same period were selected as the control group. The forced vital capacity (FV) and the forced end-expiratory volume (FEV _ 1) were measured by FVC and FEV1 at 1 seconds. The percentage of FEV1 to predicted value and FEV1 / FVC were recorded. Peripheral venous blood samples were collected from all subjects, and the polymorphism of HHIP gene was detected by im LDRTM multiplex SNP typing technique. Results the distribution of rs13118928 genotypes and alleles in the two groups were compared. There was no significant difference in the genotype and allele distribution of rs1828591 locus between the two groups. The percentage of FEV1 in patients with different genotypes of rs13118928 locus was compared with the predicted value of FEV1 / FVC.There was no significant difference between the two groups in the distribution of genotype and allele of rs13118928 locus. There was no significant difference between the two groups (P 0.05). The percentage of FEV1 in patients with different genotypes of rs1828591 locus and the percentage of FEV1 / FVC were not statistically significant. The haplotype distribution of HHIP gene rs13118928 / rs1828591 was compared between the two groups. Conclusion SNPs at rs13118928 / rs1828591 of HHIP gene has no significant relationship with COPD susceptibility of Kazak nationality in Xinjiang.
【作者单位】：石河子大学医学院第一附属医院呼吸二科;
【基金】：国家自然科学基金资助项目(81560009)
【分类号】：R563.9

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