耐碳青霉烯类抗生素肺炎克雷伯菌临床分离株中16 S rRNA甲基化酶基因的检测

发布时间：2018-02-28 14:40

本文关键词： S rRNA甲基化酶细菌耐药碳青霉烯酶肺炎克雷伯菌　出处：《中国临床药理学杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的探讨耐碳青霉烯类抗生素肺炎克雷伯菌(CRKP)临床分离株中16 S rRNA甲基化酶基因存在情况及同源性。方法收集南昌4家医院29株CRKP,用聚合酶链反应(PCR)扩增耐药基因,确定其基因型。用接合试验评价耐药质粒的可转移性,脉冲场凝胶电泳(PFGE)对其进行同源性分析。结果 29株CRKP临床分离株对阿米卡星和庆大霉素的耐药率分别为37.9%和69.0%。10株16 S rRNA甲基化酶基因阳性菌株,其中9株armA阳性,1株rmtB阳性,对阿米卡星和庆大霉素均耐药,耐阿米卡星的菌株同时耐庆大霉素。10株16 S rRNA甲基化酶基因阳性菌中均同时携带β内酰胺酶基因,其中7株同时携带碳青霉烯酶基因,以KPC-2及NDM-1为主。10株菌株均被PFGE成功分型,分别属于9个不同克隆,3株armA阳性菌通过接合实验成功的把耐药质粒传递给受体菌E.coli J53。结论 CRKP对氨基糖苷类抗生素的耐药性与16S rRNA甲基化酶基因高度有关,且armA是其主要型别。携带16 S rRNA甲基化酶基因的质粒可通过水平播散。
[Abstract]:Objective to investigate the existence and homology of 16s rRNA methylase gene in clinical isolates of Klebsiella pneumoniae resistant to carbapenem. Methods 29 strains of CRKP from 4 hospitals in Nanchang were collected and amplified by polymerase chain reaction (PCR). To determine their genotypes. To evaluate the transferability of drug-resistant plasmids by conjugation test, Results the resistance rates of 29 clinical isolates of CRKP to amikacin and gentamicin were 37.9% and 69.0.10, respectively, of which 9 were armA positive and 1 rmtB positive. The strains resistant to amikacin and gentamicin were resistant to gentamicin at the same time. All of the 10 positive strains of 16s rRNA methylase gene carried 尾 lactamase gene, and 7 of them also carried carbapenase gene. Ten strains of KPC-2 and NDM-1 were successfully typed by PFGE. Three strains of armA positive strains belonging to 9 different clones were successfully transferred to E. coli J53 by conjugation. Conclusion the resistance of CRKP to aminoglycoside antibiotics is highly related to the 16s rRNA methylase gene. The plasmid carrying 16s rRNA methylase gene can be spread horizontally.
【作者单位】：南昌大学第二附属医院检验科;
【基金】：江西省科技支撑计划基金资助项目(20112BBG70054)
【分类号】：R563.1

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