利奈唑胺耐药粪肠球菌感染患者临床分离株与定植株同源性分析

发布时间：2018-02-28 23:34

  本文关键词： 利奈唑胺 粪肠球菌 临床耐药 定植 同源性　出处：《中国感染控制杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的研究1例患者体内利奈唑胺(LZD)耐药粪肠球菌临床分离株与定植株同源性特点。方法对1例肺部感染患者分离的10株粪肠球菌(其中2株分离自尿标本,8株分离自粪便标本)进行细菌耐药分析,并通过脉冲场凝胶电泳(PFGE)确定粪肠球菌之间的同源性。结果患者进行LZD治疗前后,尿标本分离出的2株粪肠球菌均为LZD耐药株(MIC值分别为8 mg/mL,16 mg/mL),粪便中培养挑取的8株(治疗前6株,治疗后2株),其中LZD敏感4株,中介2株,耐药2株(MIC值波动在0.25~12 mg/mL)。通过PFGE分型检测提示10株粪肠球菌具有同源性。结论该例患者泌尿道和肠道检出的粪肠球菌具有同源性,提示LZD耐药肠球菌可能长期定植于患者体内,并可能发生移位导致耐药细菌感染。
[Abstract]:Objective to study the homology of clinical isolates of drug-resistant Enterococcus faecalis from a patient with LZD. Methods 10 strains of Enterococcus faecalis isolated from a patient with pulmonary infection were isolated from 10 strains of Enterococcus faecalis (including 2 strains isolated from urine). Analysis of bacterial drug resistance, The homology of Enterococcus faecalis was determined by pulsed field gel electrophoresis (PFGE). Results the patients were treated with LZD before and after treatment. The LZD values of two strains of Enterococcus faecalis isolated from urine samples were 8 mg / m L ~ (-1) and 16 mg / m ~ (-1), respectively, and 8 strains (6 strains before treatment and 2 strains after treatment) were isolated from feces, among which 4 strains were sensitive to LZD and 2 strains were mediators. The results showed that 10 strains of Enterococcus faecalis had homology by PFGE typing. Conclusion the detection of Enterococcus faecalis in urinary tract and intestinal tract in this case has homology, suggesting that LZD resistant Enterococcus may be colonized in the patient for a long time. Translocation may lead to resistant bacterial infection.
【作者单位】： 广东医科大学附属深圳市南山区人民医院深圳市内源性感染诊治研究重点实验室;
【基金】：深圳市科技创新委基础研究基金项目(JCYJ20150402152130173) 深圳市南山区科技项目(2015019,2015022)
【分类号】：R563.1
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