重症继发性肺结核患者外周血单个核细胞c-Jun水平降低

发布时间：2018-03-06 04:34

本文选题：重症肺结核　切入点：c-Jun　出处：《细胞与分子免疫学杂志》2015年05期 　论文类型：期刊论文

【摘要】：目的研究重症与轻症继发性肺结核患者c-Jun表达的差异,探讨其在重症继发性肺结核炎症反应和免疫损伤中的意义。方法用Affymetrix基因表达谱芯片筛选重症、轻症肺结核患者和正常健康者差异表达基因,并对差异基因进行生物信息学分析;用实时定量PCR(qRT-PCR)测定重症、轻症肺结核患者和健康者c-Jun相对水平;ELISA测定c-Jun在重症、轻症肺结核患者和健康者外周血单个核细胞(PBMC)中的蛋白水平。结果芯片筛选发现重症与轻症继发性肺结核患者存在大量差异表达基因,参与较多免疫反应和炎症反应通路,其中c-Jun表达下调2.27倍,参与61个信号通路;qRT-PCR结果显示,相比轻症继发性肺结核患者,c-Jun在重症继发性肺结核患者水平显著下调;ELISA检测也证实c-Jun表达在重症继发性肺结核患者有下调趋势,与芯片结果相符。结论重症与轻症继发性肺结核患者相比,c-Jun表达下调,c-Jun参与广泛的免疫反应和炎症反应通路,其下调与重症患者的免疫损伤有相关性。
[Abstract]:Objective to study the difference of c-Jun expression in patients with severe pulmonary tuberculosis and mild pulmonary tuberculosis, and to explore the significance of c-Jun expression in inflammatory reaction and immune injury in patients with severe pulmonary tuberculosis. Methods Affymetrix gene expression microarray was used to screen severe cases. The differentially expressed genes were analyzed by bioinformatics in patients with mild pulmonary tuberculosis and normal controls, and c-Jun was detected by real-time quantitative PCRqRT-PCR.The relative level of c-Jun in patients with mild pulmonary tuberculosis and healthy controls was determined by Elisa. The protein levels in PBMCs of peripheral blood mononuclear cells (PBMCs) in patients with mild pulmonary tuberculosis and healthy subjects were detected by microarray screening. Results A large number of differentially expressed genes were found in patients with severe and mild pulmonary tuberculosis and involved in more immune response and inflammatory response pathways. The expression of c-Jun was down-regulated by 2.27 fold, and the results of qRT-PCR showed that c-Jun was involved in 61 signaling pathways. Compared with the patients with mild secondary pulmonary tuberculosis, the expression of c-Jun in patients with severe secondary pulmonary tuberculosis was significantly down-regulated by Elisa, and the expression of c-Jun was down-regulated in patients with severe secondary pulmonary tuberculosis. Conclusion the down-regulated expression of c-Jun is involved in a wide range of immune response and inflammatory response pathways in patients with severe pulmonary tuberculosis and mild pulmonary tuberculosis. The down-regulation of c-Jun is related to the immune injury in patients with severe pulmonary tuberculosis.
【作者单位】：解放军医学院;解放军309医院结核病研究室;
【基金】：传染病重大科技专项(2013ZX10003-006-003-001)
【分类号】：R521

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