虫草菌粉对兔矽肺纤维化早期干预作用的研究

发布时间：2018-03-14 10:29

本文选题：矽肺　切入点：虫草菌粉　出处：《南昌大学》2013年硕士论文　论文类型：学位论文

【摘要】：背景：当前,从接触粉尘的人数、新发矽肺人数及现患病人数来讲，中国是受矽肺危害最严重的国家。矽肺发病机制至今尚未不清楚，学者们曾提出过诸多假说，尽管各个学说都有其合理之处，但都难以确切地描述其发病机制。近年来，TGF-β被大多数学者公认为是肺纤维化形成与发展的关键性细胞因子。在多种纤维增生性疾病中，TGF-β表现出共同促纤维化作用，其中TGF-β1为TGF-β家族中最重要的致纤维化的亚型，而Smad7蛋白是一种新发现的蛋白，是TGF-β-Smads信号通路的下游蛋白，，其异常表达在肺纤维化发生发展中起关键作用。已有很多研究表明TGF-β1和Smad7在肺纤维化发病过程中发挥重要的作用，本实验通过测定上述两种因子在各组兔肺组织中的表达，探讨其在矽肺纤维化发病过程中的可能机制。目的：成功复制兔矽肺模型，探讨虫草菌粉对兔矽肺纤维化形成过程中的干预作用。方法：选取30只健康雄性大耳白兔，随机均分为三组,即：1、空白对照组（BP），2、矽肺对照组（CP），3、实验干预组（EP），CP、EP组气管内注入二氧化硅粉尘悬液，BP组气管内注入相同体积的生理盐水。兔染矽尘30d后处死，计算肺重系数，右肺组织行HE染色，运用加碱水解法测定免肺组织的羟脯氨酸含量，实时荧光定量PCR法测免肺组织TGF-β1及Smad7的mRNA表达水平。结果：3组兔肺组织HE染色示：BP组正常肺组织，CP、EP组兔肺组织明显纤维化并有矽肺结节形成，显示矽肺兔模型造模成功。在三组兔肺组织中，肺重系数和羟脯氨酸的含量存在显著差异（P0.05）。 EP组与BP组间比较：免肺组织TGF-β1表达水平升高及Smad7表达水平下降（P0.02）；EP组与CP组比较：免肺组织TGF-β1表达水平下降及Smad7表达水平升高P0.05）。结论：虫草菌粉在兔矽肺纤维化形成的过程具有一定的影响，可能是通过减少TGF-β1mRNA表达,依赖于TGF-β-Smads信号通路来上调smad7mRNA，从而对兔矽肺纤维化的形成有一定抑制作用。
[Abstract]:Background: in terms of the number of people exposed to dust, the number of newly developed silicosis and the number of people suffering from silicosis, China is the most seriously affected by silicosis. The pathogenesis of silicosis is not yet clear, and scholars have put forward many hypotheses. Although each doctrine has its own reasonableness, In recent years, TGF- 尾 has been recognized by most scholars as the key cytokine in the formation and development of pulmonary fibrosis. TGF- 尾 plays a common role in promoting fibrosis in various fibroproliferative diseases. TGF- 尾 1 is the most important fibrogenic subtype of TGF- 尾 family, and Smad7 protein is a newly discovered protein, which is downstream of TGF- 尾 -Smads signaling pathway. Many studies have shown that TGF- 尾 1 and Smad7 play an important role in the pathogenesis of pulmonary fibrosis. To explore its possible mechanism in the pathogenesis of silicosis fibrosis. Aim: to establish rabbit silicosis model successfully and to investigate the effect of cordyceps powder on the formation of silicosis in rabbits. Methods: thirty healthy male rabbits were randomly divided into three groups. The rats in the intervention group were injected with the same volume of normal saline into the trachea of the EPX CPEP group and the BP group with silica dust suspension. After 30 days of exposure to silica dust, the rabbits were killed, the lung weight coefficient was calculated, and the right lung tissue was stained with HE. The content of hydroxyproline in lung tissues was determined by alkaline hydrolysis, and the mRNA expression of TGF- 尾 1 and Smad7 in lung tissues was measured by real-time fluorescence quantitative PCR. Results the lung tissues of the three groups showed obvious fibrosis and silicosis nodule formation in the normal lung tissue of the control group and the CPEP group, indicating that the silicosis rabbit model was successfully established, and in the lung tissue of the three groups, the lung tissue of the control group and the control group were observed. There was significant difference in lung weight coefficient and hydroxyproline content between EP group and BP group. The expression level of TGF- 尾 1 and Smad7 decreased in EP group compared with CP group. Compared with CP group, the expression level of TGF- 尾 1 and Smad7 increased P0.05in EP group. Conclusion: Cordyceps powder has a certain effect on the formation of silicosis in rabbits, which may be due to the decrease of TGF- 尾 1 mRNA expression and the up-regulation of smad7mRNAs by TGF- 尾 -Smads signaling pathway, which may inhibit the formation of silicosis in rabbits.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R135.2

【引证文献】