白细胞介素17G-152A基因多态性与支气管哮喘的相关性研究

发布时间：2018-03-14 17:30

本文选题：支气管哮喘　切入点：单核苷酸多态性（SNP）　出处：《大连医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：白细胞介素17（interleukin17，IL-17）由T细胞亚群——辅助性T细胞17(Thelp cell17, Th17)分泌产生。IL-17家族中的成员之一IL-17A在变应性炎症中具有重要的作用[1]，与支气管哮喘的发生、发展有关。IL-17G-152A基因多态性与自身免疫性疾病的相关性研究较多，然而对IL-17G-152A基因多态性与哮喘关系的研究甚少。本实验旨在通过检测哮喘患者外周血IL-17G-152A的基因表达，从而探讨IL-17G-152A单核苷酸多态性（single nucleotide polymorphism,SNP)在哮喘发病机制中的作用及中国东北地区汉族人群IL-17G-152A多态性(rs2275913)与哮喘发病的相关性。方法：采用病例对照研究方法，共收集103例哮喘患者和48例健康对照组。采集外周静脉血，提取全血基因组DNA，用Real-time PCR方法检测IL-17G-152A(rs2275913)SNP。计量资料以均数±标准差（x±s）表示，符合正态分布数据采用样本均数t检验。计数资料以构成比表示，，组间基因型频率等计数资料的比较采用χ~2检验，单变量Logistic回归分析探讨等位基因、基因型与哮喘发病的相关性。结果： 1. IL-17G-152A位点在中国东北地区存在多态性，各组基因型频率符合Hardy-Weinberg平衡。健康人群A、G等位基因频率分别45.8％、54.2％；AA、AG和GG基因型频率分别20.8％、50％、29.2％。 2．哮喘组IL-17G-152A位点等位基因A（χ~2=0.108，P=0.742＞0.05）及AA基因型频率（χ~2=0.977，P=0.614＞0.05）与正常对照组比较无统计学差异。 3．外周血嗜酸性粒细胞、血清总IgE、基础肺功能FEV1/pred%、FEV1/FVC%异常个体在各基因型组间比较差异无统计学意义（P＞0.05）。 4.单变量Logistic回归分析探讨等位基因、基因型与哮喘发病的相关性，P值均＞0.05，对疾病均没有显著性影响。回归模型对疾病变异的贡献是6%。结论： 1. IL-17G-152A位点在中国东北地区汉族人群中存在多态性。 2. IL-17G-152A单核苷酸多态性与中国东北地区哮喘发病无关，未发现A等位基因及AA基因携带者罹患哮喘的风险增加，即rs2275913SNP并非哮喘的易感位点。 3.IL-17G-152A基因多态性与外周血嗜酸性粒细胞、血清总IgE、肺功能FEV1/pred%、FEV1/FVC%异常个体的比例无明显相关性。
[Abstract]:Objective:. Interleukin 17 interleukin 17 (IL-17) is secreted by T cell subsets, helper T cell 17T help cell 17 (Th17) to produce IL-17A, one of the members of the IL-17 family, which plays an important role in allergic inflammation [1], and is associated with the occurrence of bronchial asthma. The relationship between the polymorphism of IL-17G-152A gene and autoimmune diseases was studied, but the relationship between the polymorphism of IL-17G-152A gene and asthma was not studied. The aim of this study was to detect the gene expression of IL-17G-152A in peripheral blood of asthmatic patients. To explore the role of single nucleotide polymorphisms (IL-17G-152A) in the pathogenesis of asthma and the relationship between IL-17G-152A polymorphism rs2275913) and asthma. Methods:. Using a case-control study, 103 asthmatic patients and 48 healthy controls were collected. Peripheral venous blood was collected and genomic DNA was extracted from the whole blood. IL-17G-152Atran rs2275913 SNPs were detected by Real-time PCR. The measured data were expressed as mean 卤standard deviation (x 卤s). According to the normal distribution data, the sample mean t test was used. The counting data were expressed as composition ratio, the genotypes frequency and other counting data were compared by 蠂 ~ 2 test, and the alleles were analyzed by univariate Logistic regression analysis. Relationship between genotype and asthma. Results:. 1. The polymorphism of IL-17G-152A locus was found in Northeast China, and the allele frequencies of AHG in each group were consistent with Hardy-Weinberg balance. The allele frequencies of AHG in healthy population were 45.8and 54.2GG, respectively. The frequencies of AA AG and GG genotype were 20.850 and 29.229.2 respectively. 2. There was no significant difference in the allele A of IL-17G-152A locus between asthma group and normal control group (蠂 2 + 0.108) and the frequency of AA genotype (蠂 2 + 0.97 7 P0. 614 > 0. 05). 3.Peripheral blood eosinophilic granulocytes, serum total IgE, FEV 1 / pred% abnormal individuals had no significant difference among the genotypes (P > 0.05). 4. Univariate Logistic regression analysis was used to study alleles. The correlation between genotype and asthma was more than 0.05, which had no significant effect on disease. The contribution of regression model to disease variation was 6%. Conclusion:. 1. The polymorphism of IL-17G-152A locus was found in the Han population in Northeast China. 2. The single nucleotide polymorphisms of IL-17G-152A were not associated with asthma in Northeast China. There was no increased risk of asthma in A allele and AA gene carriers, that is, rs2275913SNP was not a susceptible site for asthma. 3. The polymorphism of IL-17G-152A gene had no significant correlation with the proportion of individuals with abnormal FEV 1 / FVC% in peripheral blood eosinophil, serum total IgE and pulmonary function FEV 1 / pred.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R562.25

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