氨溴索注射液对大鼠气管内注药所致气管黏膜损伤的保护作用

发布时间：2018-03-19 00:11

本文选题：气管内给药　切入点：气管黏膜　出处：《福建医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：研究气管内注射阿米卡星对Wistar大鼠气管黏膜的超微结构和病理的影响，并利用电镜和光镜分别观察氨溴索注射液干预处理后对阿米卡星组大鼠气管黏膜变化的影响，进一步了解埅痰药氨溴索在黏液纤毛系统中的作用。材料与方法：将30只雄性Wistar大鼠随机分为2组：阿米卡星组、氨溴索干预组各15只。对照组（阿米卡星组）及氨溴索干预组，各15只大鼠。各组大鼠麻醉后均经气管穿刺注入阿米卡星0.252mL/kg，氨溴索干预组在气管注入阿米卡星后立即腹腔注射氨溴索注射液70mg/kg,分别于给药后第2、4、8、24、48小时处死大鼠，解剖分离气管及肺组织，扫描电镜观察支气管黏膜的超微结构变化特点，用Image-ProPlus6.0图像分析软件对每张扫描电镜图片进行半定量分析纤毛受损面积，并观察右肺组织病理变化。结果：1、电镜下阿米卡星组大鼠气管黏膜均出现不同程度的纤毛受损，表现为排列紊乱、倒伏、粘着、变短，部分断裂缺失，伴不同程度的黏液分泌增加。光镜下阿米卡星组大鼠肺部出现纤毛紊乱，黏液分泌增加、杯状细胞增多，部分支气管肺组织内可见炎症细胞浸润。2、阿米卡星组比氨溴索干预组大鼠纤毛受损面积增加（P0.01）。3、在各个相应的时间点，阿米卡星组纤毛受损面积均大于氨溴索干预组（P0.01）；氨溴索组纤毛结构恢复较好。4、阿米卡星组受损面积组内比较中，第2h、4h受损面积相比无显著差异（P0.05），第2h、4h明显高于第8h（P0.01），第8h大于第24h（P0.01），第24h高于第48h（P0.01）；氨溴索干预组受损面积组内比较中，第4h和2h相比有减少趋势（P0.01），第4h明显高于8h（P0.01），，第8h明显高于24h（P0.01），第24h明显高于48h（P0.01）。5、两组纤毛受损面积组间相互比较中，第2h阿米卡星组受损面积大于氨溴索干预组（P0.01）；第4h、8h、24h、48h阿米卡星组受损面积大于氨溴索干预组（P0.01）。结论：1、气管内局部注射阿米卡星，均可对大鼠气管黏膜表面纤毛超微结构和肺脏细支气管黏膜造成不同程度的急性损伤，但损伤具有可逆性，随时间增加自我修复。2、氨溴索干预后气管内注入阿米卡星大鼠虽然纤毛结构受损，但程度较阿米卡星组明显减轻，病理提示肺部炎症细胞少见，提示氨溴索注射液可促进纤毛结构修复，并减轻肺部炎症反应，缩短恢复时间。
[Abstract]:Objective: to study the effects of intratracheal injection of amikacin on the ultrastructure and pathology of tracheal mucosa in Wistar rats. To further understand the role of ambroxol in mucociliary system. Materials and methods: thirty male Wistar rats were randomly divided into two groups: amikacin group, ambroxol intervention group (15 rats), control group (amikacin group) and ambroxol intervention group (ambroxol group). The rats in each group were injected with amikacin 0.252 mL / kg by trachea puncture after anesthesia, and ambroxol injection of 70 mg / kg was injected intraperitoneally in the ambroxol intervention group immediately after trachea injection. The trachea and lung tissues were dissected and the ultrastructural changes of bronchial mucosa were observed by scanning electron microscope (SEM). The damage area of cilium was semi-quantitatively analyzed by Image-ProPlus6.0 image analysis software, and the pathological changes of right lung tissue were observed. Results under the electron microscope, the mucous membrane of the trachea of amicacin group showed various degree of cilia damage, such as disorder of arrangement, lodging, adhesion, shortening, partial rupture and deletion. With the increase of mucus secretion in different degree. Under light microscope, amikacin group had ciliated disorder, increased mucus secretion and goblet cells. Inflammatory cell infiltration could be seen in some bronchopulmonary tissues. Amikacin group could increase the damaged area of cilium in ambroxol group compared with ambroxol group. The damaged area of cilia in amikacin group was larger than that in ambroxol intervention group (P 0.01), the cilium structure of ambroxol group recovered better than that of ambroxol group, and that of amikacin group was better than that of ambroxol group. There was no significant difference in the damaged area between the 2nd hour and the 4th hour (P 0.05), but at the second hour, it was significantly higher than that in the 8th hour (P 0.01), in the 8th hour was higher than that in the 24 h (P 0.01), and in the 24 h was higher than that in the 48 h (P 0.01), and in the ambroxol intervention group, the damaged area was higher than that in the control group. At the 4th hour and the 2nd hour, there was a decreasing trend of P0.01U, the 4th hour was significantly higher than the 8h P0.01U, the 8h was significantly higher than the 24h P0.01U, the 24h was significantly higher than the 48hu P0.01U 路5. in the comparison between the two groups, the area of the damaged cilium in the two groups was significantly higher than that in the control group. The damaged area of amikacin group at 2 h was larger than that of ambroxol intervention group (P 0.01), and the damage area of amikacin group was larger than that of ambroxol intervention group (P 0.01). ConclusionAmikacin injected locally into trachea can cause different degrees of acute injury to the surface ciliated ultrastructure of trachea mucosa and bronchiolus mucosa in rats, but the injury is reversible. After the intervention of ambroxol, although the cilium structure was damaged, the degree of amikacin group was much less than that of amikacin group, and the pathological results showed that the inflammatory cells of lung were rare. The results suggest that ambroxol injection can promote ciliated structure repair, alleviate pulmonary inflammation and shorten recovery time.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R562

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