褪黑素对矽肺大鼠肺纤维化的影响

发布时间：2018-03-22 01:20

本文选题：褪黑素　切入点：矽肺　出处：《环境与职业医学》2017年07期 　论文类型：期刊论文

【摘要】：[目的]研究褪黑素(melatonin,MT)对矽肺大鼠肺纤维化的影响及其可能机制。[方法]将72只SPF级SD雄性大鼠,随机分为4组(对照组、MT组、染尘组及MT+染尘组),每组18只。对照组及MT组采用气管插管灌注法,一次性气管内注入1 m L生理盐水,其他两组采用同样方法注入50 mg/m L的二氧化硅悬液1 m L。每天给予MT组、MT+染尘组腹腔注射MT(10 mg/kg),而染尘组及对照组给予腹腔注射同体积生理盐水。每组分别于染毒后第7、14、28天随机处死6只大鼠。观察大鼠的肺脏器系数、肺组织形态,测定肺组织羟脯氨酸(Hyp)含量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、肺组织核转录因子-κB(NF-κB)的表达。[结果]MT+染尘组大鼠肺泡炎症细胞浸润、充血水肿、肺间质纤维化的程度较染尘组相比有明显改善;与同期染尘组大鼠相比,MT+染尘组大鼠肺脏器系数、Hyp含量、MDA含量,SOD活性增加,差异均有统计学意义(均P0.05)。Western blot检测结果显示,MT+染尘组大鼠肺组织NF-κB蛋白表达量(0.36±0.02)低于染尘组(0.48±0.01),差异有统计学意义(P0.05)。[结论]褪黑素对矽肺大鼠肺纤维化有保护作用,其作用机制可能是通过改善机体氧化/抗氧化系统的失衡、抗炎,从而减少纤维化的形成。
[Abstract]:[objective] to study the effect of melatonin on pulmonary fibrosis in silicosis rats and its possible mechanism. [methods] Seventy-two SPF male SD rats were randomly divided into 4 groups (control group). There were 18 rats in each group in Dust group and MT group. The control group and MT group were infused with tracheal intubation, and 1 mL saline was injected into the trachea at one time. The other two groups were injected with 50 mg/m / L silica suspension 1 mL / day. The MT group was given intraperitoneal injection of MT(10 mg / kg of MT(10 mg / kg per day, while the dust-infected group and the control group were given intraperitoneal injection of normal saline of the same volume. Six rats were killed at random on the 28th day of the 7th day, and the lung organ coefficients of the rats were observed. The lung tissue morphology, the content of hydroxyproline, the activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the expression of nuclear transcription factor 魏 B (NF- 魏 NF- 魏 B) in lung tissue were measured. [results] the alveolar inflammatory cells infiltrated and hyperemia and edema were observed in MT dusty rats. The degree of pulmonary interstitial fibrosis was significantly improved than that of dust-exposed group, and the content of Hyp content and MDA content and SOD activity in lung tissue were increased in MT dust-exposed group compared with that in dust-exposed group. The expression of NF- 魏 B protein in lung tissue of rats exposed to MT dust was 0.36 卤0.02, which was significantly lower than that of dust-exposed group (0.48 卤0.01). [conclusion] melatonin has protective effect on pulmonary fibrosis in silicosis rats. The mechanism may be to reduce the formation of fibrosis by improving the imbalance of oxidation / antioxidant system and anti-inflammation.
【作者单位】：徐州医科大学第二附属医院;
【分类号】：R135.2;R563

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