急性呼吸窘迫综合征大鼠血小板MKK3磷酸化水平变化初探

发布时间：2018-03-23 14:00

本文选题：急性呼吸窘迫综合征　切入点：血小板　出处：《中国呼吸与危重监护杂志》2017年01期

【摘要】：目的探讨急性呼吸窘迫综合征(ARDS)发病的分子机制以及血小板活化的信号通路。方法将30只健康SD大鼠随机分为5组。其中实验组4组,尾静脉注射油酸(0.25 ml/kg)制备ARDS模型;对照组1组,尾静脉注射等量生理盐水。在实验组注射油酸后2、6、24、72 h,对照组注射生理盐水后2 h,处死大鼠,从腹主动脉采血,分离血小板,提取血小板蛋白,用免疫印迹法检测动物血小板内丝裂原活化蛋白激酶激酶3(MKK3)的磷酸化水平变化,了解ARDS时血小板丝裂原活化蛋白激酶(MAPKs)信号通路的改变以及与ARDS发病的关系。结果注射油酸后6~72 h大鼠血小板的MKK3磷酸化水平明显增高(P0.05),其中6 h组为对照组的2.4倍(0.50±0.09 vs.0.21±0.05);24 h组表达量最高(0.78±0.06),为对照组的3.7倍;72 h组稍有回落(0.75±0.13),但仍明显高于对照组。结论 ARDS时血小板的活化过程与MKK3-p38 MAPK信号转导通路的启动有关。
[Abstract]:Objective to investigate the molecular mechanism of acute respiratory distress syndrome (ARDS) and the signal pathway of platelet activation. Methods Thirty healthy SD rats were randomly divided into five groups: experimental group (n = 4) and caudal vein injection of oleic acid 0.25 ml / kg to establish ARDS model. The rats in the control group were killed 2 hours after the injection of oleic acid, the rats were killed at 2 h after injection of oleic acid, the blood was collected from the abdominal aorta, platelets were isolated and platelet protein was extracted. The phosphorylation of mitogen-activated protein kinase 3 (MKK3) in animal platelets was detected by Western blot. To investigate the changes of mitogen-activated protein kinase (MAPKs) signaling pathway in platelets during ARDS and its relationship with the pathogenesis of ARDS. Results the level of MKK3 phosphorylation in platelets of rats at 6h after oleic acid injection was significantly higher than that of control group (P 0.05). The highest expression level was 0.78 卤0.06 in the 24 h group with 0.50 卤0.09 vs.0.21 卤0.05 vs.0.21 卤0.05, which was a little lower than that in the control group at 72 h, but still significantly higher than that in the control group. Conclusion the activation process of platelet during ARDS is related to the initiation of MKK3-p38 MAPK signal transduction pathway.
【作者单位】：山西大同大学呼吸病与职业病研究所;临汾市中心医院呼吸科;
【基金】：山西省自然科学基金(2013011055-5) 大同市基础研究项目(2014105-2)
【分类号】：R563.8

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