慢性阻塞性肺疾病患者静脉血CD40、CD86和GSTO1变化及意义

发布时间：2018-03-29 18:21

本文选题：树突细胞　切入点：还原型谷胱甘肽硫转移酶-　出处：《中国老年学杂志》2014年18期

【摘要】：目的分析CD40、CD86和还原型谷胱甘肽硫转移酶(GSTO)1在慢性阻塞性肺疾病(COPD)发病机制中的作用。方法以COPD患者为研究对象,以健康人作为对照,采集空腹静脉血,对CD40、CD86变化实施流式细胞术(FCM)检测,对GSTO1含量采用Western印迹检测。结果相比于非COPD(吸烟无COPD、健康无吸烟者)人群,COPD患者外周血中CD40、CD86增高明显,GSTO1降低明显(P0.05);COPD患者CD40、CD86与肺功能呈负相关(P0.05),而GSTO1与COPD患者肺功能呈正相关(P0.05),GSTO1与CD40、CD86呈负相关(P0.05)。结论树突细胞在COPD发生发展中起着重要作用;COPD患者体内存在氧化应激,并与病情密切相关;树突细胞成熟可能被氧化应激反应激发。
[Abstract]:Objective to analyze the role of CD40 CD86 and reduced glutathione S-transferase (GSTO-1) in the pathogenesis of chronic obstructive pulmonary disease (COPD). Methods fasting venous blood was collected from COPD patients and healthy controls. The changes of CD40 and CD86 were detected by flow cytometry (FCM). The content of GSTO1 was detected by Western blot. Results compared with non-COPD (smoking non-COPD-healthy non-smoker) patients, CD40T CD86 in peripheral blood of patients with GSTO1 was significantly higher than that in patients with GSTO1. CD40T CD86 was negatively correlated with pulmonary function in patients with GSTO1, and the pulmonary function of patients with GSTO1 and COPD was negatively correlated with that of patients with COPD. Conclusion dendritic cells play an important role in the occurrence and development of COPD, and oxidative stress exists in the body of patients with COPD, and the relationship between GSTO1 and CD40 / CD86 is negatively correlated with that of GSTO1.Conclusion dendritic cells play an important role in the pathogenesis and development of COPD. The maturation of dendritic cells may be stimulated by oxidative stress.
【作者单位】：遵义医学院附属医院呼吸一科;
【基金】：贵州省科技厅社发攻关项目〔黔科合SY字(2009)3053〕
【分类号】：R563.9

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