VEGF在良恶性胸腔积液中表达差异的研究

发布时间：2018-04-02 21:54

本文选题：血管内皮生长因子　切入点：胸腔积液　出处：《天津医科大学》2012年硕士论文

【摘要】：目的：通过检测结核性胸膜炎及恶性胸腔膜炎患者血清和胸水中VEGF含量, 探讨VEGF在结核性胸膜炎及恶性胸膜炎患者血清、胸水中表达的差异,并分析VEGF在不同原因胸膜炎的胸水和血清中是否存在相关性。方法：以经可弯曲电子胸腔镜下取胸膜活检经病理确诊的结核性胸膜炎和恶性胸膜炎患者各30例为研究对象,对所有患者在进行可弯曲电子胸腔镜检查术中留取胸水标本10ml,并在手术当天取患者静脉血5m1标本,采用双抗体夹心酶联免疫吸附实验(ELISA)检测患者胸水及血清中VEGF水平。两组患者血清和胸水VEGF差异比较采用t检验,两组患者胸水与血清VEGF相关分析采用直线相关及逐步回归分析。结果：结核性胸膜炎患者血清和胸水VEGF检测值分别为45.33±18.33pg/mL,62.73±24.65pg/mL恶性胸膜炎血清和胸水VEGF检测值分别为66.00±29.83pg/mL,95.54±42.11pg/mL。将结核性胸膜炎组与恶性胸膜炎组的血清、胸水中的VEGF含量进行比较,胸水中VEGF含量均高于血清中的含量(t值分别为2.61,3.315,均P0.05),差异有统计学意义。将恶性胸膜炎和结核性胸膜炎患者的血清、胸水中VEGF含量进行比较,恶性胸膜炎患者的血清及胸水中VEGF含量均高于结核性胸膜炎患者(t值分别为3.9,5.2,均P0.05),差异有统计学意义。对结核性胸膜炎患者与恶性胸膜炎患者血清和胸水VEGF检测值进行相关性分析(R值分别为0.53,0.38,均P0.05),VEGF在结核性胸膜炎和恶性胸膜炎的血清和胸水中均呈正相关性。结论：VEGF在结核性胸膜炎及恶性胸膜炎患者血清、胸水中的表达有差异,恶性高于结核性；两组患者胸水中VEGF含量均高于血清,胸水VEGF含量随着血清VEGF含量增高而增高。检测胸腔积液患者血清和胸水中VEGF含量对结核性胸膜炎和恶性胸膜炎的诊断和鉴别诊断有一定的价值。
[Abstract]:Objective: to detect the content of VEGF in serum and pleural effusion in patients with tuberculous pleurisy and malignant pleurisy.To investigate the difference of VEGF expression in serum and pleural effusion of patients with tuberculous pleurisy and malignant pleurisy, and to analyze the correlation between VEGF in pleural effusion and serum of different causes pleurisy.Methods: 30 cases of tuberculous pleurisy and 30 cases of malignant pleurisy confirmed pathologically by flexible electronic thoracoscopy were studied.Samples of pleural effusion were collected from all patients during the operation of bendable electron thoracoscopy, and venous blood 5m1 samples were collected on the day of operation. The VEGF levels in pleural effusion and serum were detected by double antibody sandwich enzyme-linked immunosorbent assay (Elisa).The difference of VEGF between serum and pleural effusion was analyzed by t test. Linear correlation and stepwise regression analysis were used to analyze the correlation between pleural effusion and serum VEGF in the two groups.Results: the detection values of VEGF in serum and pleural effusion of tuberculous pleurisy patients were 45.33 卤18.33pg / mL0.73 卤24.65pg/mL and 66.00 卤29.83pg / mL, respectively. The detection values of VEGF were 95.54 卤42.11pgmLrespectively.The contents of VEGF in serum and pleural effusion of tuberculous pleurisy group and malignant pleurisy group were compared. The VEGF content in pleural effusion was higher than that in serum was 2.61 卤3.315, respectively (P 0.05). The difference was statistically significant.The contents of VEGF in serum and pleurisy of patients with malignant pleurisy and tuberculous pleurisy were compared. The VEGF content in serum and pleurisy of malignant pleurisy patients was higher than that in tuberculous pleurisy patients (P 0.05).The correlation analysis of serum and pleural effusion VEGF detection between patients with tuberculous pleurisy and malignant pleurisy was carried out. The R value was 0. 53 卤0. 38, respectively. There was a positive correlation between serum and pleural effusion of tuberculous pleurisy and malignant pleurisy.Conclusion the expression of VEGF in pleural effusion of tuberculous pleurisy and malignant pleurisy is higher than that of tuberculous pleurisy, and the content of VEGF in pleural effusion is higher than that in serum, and the content of VEGF in pleural effusion increases with the increase of serum VEGF content.Detection of VEGF in serum and pleural effusion is valuable in the diagnosis and differential diagnosis of tuberculous pleurisy and malignant pleurisy.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R561.3

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