慢性阻塞性肺疾病患者外周血单个核细胞丝裂原活化蛋白激酶p38的表达

发布时间：2018-04-16 12:21

本文选题：p + MAPK　；参考：《中国老年学杂志》2016年12期

【摘要】：目的研究丝裂原活化蛋白激酶p38(p38 MAPK)在慢性阻塞性肺疾病(COPD)患者外周血单个核细胞中的表达情况及其与气流受限的相关性。方法选择确诊的急性加重期COPD(AECOPD)患者50例作为实验组,根据肺功能检测,分为重-极重度组(A组)26例和轻-中度组(B组)24例。选择同期门诊健康受试者25例为对照组,采集各组受试者外周血,运用PCR荧光检测法测定P38 MAPK mRNA的表达,酶联免疫吸附法(ELISA)检测外周血白细胞介素(IL)-6和IL-8含量。结果实验组患者外周血单个核细胞核内p38 MAPK mRNA表达和血清IL-6、IL-8含量均高于对照组(P0.05);A组患者外周血单个核细胞核内p38 MAPK mRNA的表达和血清IL-6、IL-8含量显著高于B组(P0.05);AECOPD患者外周血单个核细胞核内p38 MAPK mRNA表达随1 s用力呼气容积(FEV1)占预计值升高而下降(r=-0.91,P0.05)。结论 p38 MAPK、IL-6、IL-8参与了COPD的炎症反应,且同COPD患者的气流受限程度呈正相关。
[Abstract]:Objective to investigate the expression of mitogen-activated protein kinase (p38(p38) in peripheral blood mononuclear cells (PBMC) of patients with chronic obstructive pulmonary disease (COPD) and its correlation with airflow limitation.Methods 50 patients with acute exacerbation were selected as experimental group. According to the pulmonary function test, they were divided into two groups: group A (n = 26) and group B (n = 24).In the same period, 25 healthy subjects were selected as control group. Peripheral blood samples were collected from each group. The expression of P38 MAPK mRNA was detected by PCR fluorescence assay, and the levels of IL-6 and IL-8 in peripheral blood were detected by enzyme linked immunosorbent assay (Elisa).Results the expression of p38 MAPK mRNA in peripheral blood mononuclear nuclei and the level of serum IL-6 IL-8 in peripheral blood mononuclear nuclei of patients in the experimental group were higher than those in the control group P0.05AECOPD patients. The expression of p38 MAPK mRNA in the peripheral blood mononuclear nuclei and the level of serum IL-6 IL-8 in the peripheral blood mononuclear nuclei of patients with AECOPD were significantly higher than those in the patients with P0.05AECOPD.The expression of p38 MAPK mRNA in peripheral blood mononuclear cells decreased with the increase of forced expiratory volume (FEV1).Conclusion p38 MAPK- IL-6 IL-8 is involved in the inflammatory reaction of COPD, and is positively correlated with the degree of airflow limitation in COPD patients.
【作者单位】：川北医学院附属医院呼吸内科;宁夏医科大学;
【基金】：国家自然科学基金项目(No.81260569) 四川省教育厅基金项目(No.13ZA0216)
【分类号】：R563.9

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