PM2.5吸入对哮喘大鼠气道炎症的影响

发布时间：2018-04-21 08:34

本文选题：哮喘大鼠 + PM2.5　；参考：《吉林大学》2017年硕士论文

【摘要】：目的:PM2.5对哮喘大鼠气道炎症和体重的影响进行研究。方法:将124只雄性SD大鼠随机分为4组:A(正常组)、B(哮喘未吸入PM2.5组)、C1(哮喘吸入低剂量PM2.5组)、C2(哮喘吸入高剂量PM2.5组)。模型大鼠给予卵清蛋白腹腔注射两次致敏,2周后再用卵清蛋白连续雾化吸入14天,建立大鼠过敏性哮喘模型。PM2.5模型组,每周一至周三连续3天,每天1次,每次20分钟超声雾化吸入PM2.5。在吸入PM2.5一周、二周、四周、六周后观察各组大鼠哮喘发作表现和体重情况。雾化结束1天后取材,进行BALF白细胞计数和分类,ELISA试剂盒检测血清总IgE,BALF中IL-6、IL-8含量。结果:1.成功建立SD大鼠哮喘模型。2.PM2.5对哮喘大鼠体重的影响B组大鼠在建模期、染毒一周和染毒二周时与A组比较有显著差异,其中在建模期和染毒一周时差异非常显著(P0.01),染毒二周时P0.05;C2组在建模期、染毒期各个时间段与A组比较均有显著差异(P0.01),且在染毒四周、六周时与B组比较有显著差异,染毒四周时差异更显著(P0.01);C1组结果与C2组结果相似,两组的差异仅在于后者染毒二周时与B组比较有统计学差异。3.PM2.5对哮喘大鼠BALF中白细胞计数和分类的影响染毒第一周,C1组、C2组BALF中白细胞总数均明显低于B组(均p 0.01);C2组:嗜碱细胞百分比明显低于A组、B组和C1组;淋巴细胞百分比明显高于B组和A组;C1组:淋巴细胞百分比明显高于B组。染毒第二周,C1组及C2组白细胞总数均明显低于A组,白细胞分类各组间无统计学差异。染毒第四周,C1组中性粒细胞百分比明显高于A组和B组;C1组和C2组:嗜酸细胞百分比明显低于A组。染毒第六周,C2组中性粒细胞百分比明显高于A组、B组和C1组。4.PM2.5对哮喘大鼠血清IgE、BALF中IL-6、IL-8含量的影响染毒第一周,BALF中IL-8含量C1组明显低于A组和B组,C2组明显低于B组,但明显高于C1组。染毒第二周,血清IgE、BALF中IL-6、IL-8含量无统计学差异。染毒第四周,C1组BALF中IL-6含量明显低于A组;IL-8含量C1组和C2组均明显低于A组和B组。染毒第六周,C1组大鼠血清IgE含量出现明显升高,明显高于A组和B组;BALF中IL-6含量C1组明显高于A组。不同染毒时间段血清IgE含量,C1组:染毒第四周明显低于第二周,染毒第六周又明显高于第一周和第四周;C2组:染毒第六周明显高于第四周。结论:1.PM2.5能使哮喘大鼠体重明显下降,染毒第六周,吸入PM2.5的哮喘大鼠体重也未能达到正常大鼠和哮喘大鼠体重水平。2.染毒第一周:PM2.5能加重哮喘大鼠气道炎症,并且与PM2.5剂量相关,气道炎症以淋巴细胞反应为主;染毒四周以后,PM2.5导致的气道炎症加重,以中性粒细胞反应为主;整个染毒期间嗜酸细胞和嗜碱性粒细胞在气道炎症反应中不起主要作用。3.PM2.5使哮喘大鼠BALF中IL-8、IL-6含量均出现明显变化,尤其是吸入低剂量PM2.5组更明显,并且变化幅度IL-8远大于IL-6,其出现变化时间也明显早于IL-6。4.染毒第六周,PM2.5使哮喘大鼠血清IgE含量出现明显增高,早期PM2.5吸入未启动IgE介导的哮喘炎症反应途径。
[Abstract]:Objective: To study the effect of PM2.5 on airway inflammation and body weight in asthmatic rats. Methods: 124 male SD rats were randomly divided into 4 groups: A (normal group), B (asthma uninhaled PM2.5 group), C1 (asthma inhaled low dose PM2.5 group), C2 (asthma inhaled high dose PM2.5 group). Model rats were given ovalbumin intraperitoneal two times sensitization, and then ovalbumin after 2 weeks. The.PM2.5 model group was set up for 14 days after inhalation of the protein, and the model group was established for 3 days from Monday to Wednesday, 1 times a day. PM2.5. inhaled by ultrasonic atomization at 20 minutes each time was inhaled for one week, two weeks, four weeks, and six weeks to observe the symptoms and body weight of the rats in each group. After the atomization, the materials were obtained for 1 days and BALF white blood cells were obtained. Count and classify the total IgE of serum, IL-6 and IL-8 in BALF. Results: 1. the effect of.2.PM2.5 on the weight of asthma rat model of SD rats was successfully established in the modeling period of group B, and there was a significant difference between the group of B rats and the group of A for one week and two weeks, and the difference between the modeling period and the one week was very significant (P0.01). (P0.01). 1. At two weeks of poison, there were significant differences between group C2 and group A in the modeling period (P0.01), and there was a significant difference between the group of A and the B group at the time of exposure, and at six weeks, the difference was more significant (P0.01). The result of the C1 group was similar to that of the C2 group. The difference between the two groups was that the latter was only compared with the B group when compared with the B group. The effect of.3.PM2.5 on leukocyte count and classification of BALF in asthmatic rats for the first week, the total number of white blood cells in group C1 and C2 was significantly lower than that in group B (P 0.01); C2 group: the percentage of basophils was significantly lower than that of the A group, B group and C1 group; the percentage of lymphocytes was significantly higher than that in the B group and the group; the percentage of lymphocytes was significantly higher than that of the group. Second weeks, the total number of white blood cells in group C1 and C2 group was significantly lower than that of group A. There was no statistical difference between groups of white blood cells. The percentage of neutrophils in group C1 was significantly higher than that in group A and B group; the percentage of eosinophils in group C1 and C2 group was significantly lower than that in group A. The percentage of neutrophils in C2 group was significantly higher than that in A group. The effect of PM2.5 on the serum IgE, IL-6 and IL-8 content in the serum of the asthmatic rats was infected for the first week. The IL-8 content in C1 group was obviously lower than that of the A group and B group in the BALF group, and the C2 group was obviously lower than that of the B group, but the content of the serum was significantly higher than that in the group of B. The content of the serum was significantly lower than that in the group. The content of the serum was significantly lower than that in the group. The content of IL-8 in the second weeks was significantly lower than that in the group. 1 groups and C2 groups were significantly lower than group A and group B. The serum IgE content in C1 group was obviously higher than that of group A and B group. The IL-6 content of C1 group in BALF was significantly higher than that in A group. Group: sixth weeks was significantly higher than that of the fourth week. Conclusion: 1.PM2.5 can reduce the weight of asthmatic rats significantly, and the body weight of asthmatic rats can not be reduced significantly. The body weight of the asthmatic rats inhaled PM2.5 can not reach the normal rats and the weight level.2. of the asthmatic rats for the first week. PM2.5 can aggravate the airway inflammation in the asthmatic rats, and it is related to the dose of PM2.5, the airway inflammation is drenched. After four weeks of exposure, PM2.5 resulted in increased airway inflammation and neutrophil reaction. During the whole exposure, eosinophils and basophils did not play a major role in airway inflammation,.3.PM2.5 made the BALF IL-8 and IL-6 content of the asthmatic rats significantly changed, especially in the low dose PM2.5 group. More obviously, the amplitude of IL-8 was much greater than that of IL-6, and its change time was obviously earlier than that of IL-6.4. for sixth weeks. PM2.5 increased the serum IgE content in the asthmatic rats, and the early PM2.5 inhalation did not start the IgE mediated asthma inflammatory response.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R562.25

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