地塞米松对支气管哮喘大鼠气道重塑及白介素-8、表皮生长因子表达的影响

发布时间：2018-04-22 22:15

本文选题：地塞米松 + 支气管哮喘　；参考：《山西医科大学》2012年硕士论文

【摘要】：目的：探讨支气管哮喘大鼠气道重塑与其血清白介素-8（interleukin-8）、肺组织表皮生长因子（Epidermal Growth Factor,EGF）表达的关系及地塞米松早、晚期的干预效果。方法： 40只清洁级wistar雄性大鼠，随机分为4组：模型组（N组）、对照组（C组）、地塞米松早期干预组(E组)、地塞米松晚期干预组(L组)，每组各10只，用10%卵蛋白(Ovalbumin，，OVA)抗原混悬液腹腔注射致敏，1%卵清白蛋白雾化诱喘，以制备大鼠哮喘气道重塑模型。其中地塞米松早、晚期干预组在不同时期给予地塞米松进行干预。而对照组大鼠用生理盐水代替卵蛋白致敏和诱喘。ELISA法测血清中IL-8含量，肺组织切片HE染色，计算机图像分析气道形态学参数，衡量气道重塑程度，免疫组化法测定肺组织中EGF含量。数据分析用SPSS17.0统计软件处理。结果：除C组外，其余各组均有气道重塑的病理改变；与C组大鼠相比较，各组大鼠气道重塑程度较重、血清IL-8浓度升高、肺组织EGF表达上调(P0.05)；与N组大鼠相比较，各组大鼠均有气道重塑程度较轻、血清IL-8浓度下降、肺组织EGF表达下调(P0.05)；与E组相比较，L组和N组大鼠气道重塑程度较重、血清IL-8浓度升高、肺组织EGF表达上调(P0.05)；另外，各组大鼠血清IL-8浓度、肺组织EGF表达与气道重塑程度相关(P0.001)。结论：在大鼠支气管哮喘气道重塑模型中证实地塞米松可以有效抑制气道重塑，且早期应用更能尽早阻断其病程的进展；同时地塞米松可以下调IL-8、EGF的表达，而且IL-8、EGF含量与气道重塑程度呈正相关，由此推测地塞米松可能是通过抑制IL-8、EGF表达的途径来干预气道重塑，另外IL-8、EGF也可能作为衡量气道重塑程度重要指标之一。
[Abstract]:Objective: To investigate the relationship between airway remodeling and the expression of interleukin-8 in serum and epidermal Growth factor-EGFin in lung tissue of asthmatic rats and the effect of dexamethasone on the early and late stage of dexamethasone. Methods: Forty clean grade wistar male rats were randomly divided into four groups: model group (n group), control group (group C), dexamethasone early intervention group (group E) and dexamethasone late intervention group (group L) with 10 rats in each group. The airway remodeling model of asthmatic rats was established by injecting 10% ovalbumin OVA antigen suspension intraperitoneally with 1% ovalbumin and 1% ovalbumin to induce asthma. Dexamethasone in early and late intervention group was given dexamethasone in different periods. In the control group, the rats were sensitized by normal saline instead of ovalbumin and panting. Elisa was used to measure the content of IL-8 in serum, HE staining in lung tissue sections, morphological parameters of airway in computer image analysis, and the degree of airway remodeling. The content of EGF in lung tissue was determined by immunohistochemical method. Data analysis is processed by SPSS17.0 statistical software. Results: There were pathological changes of airway remodeling in all groups except group C. compared with group C, the degree of airway remodeling was heavier, the concentration of serum IL-8 was higher, the expression of EGF in lung tissue was up-regulated (P0.05), and compared with group N, the degree of airway remodeling in each group was higher than that in group C. Compared with group E, the degree of airway remodeling in group L and group N was more severe, the concentration of serum IL-8 was higher, and the expression of EGF in lung tissue was up-regulated (P 0.05). The concentration of serum IL-8 and the expression of EGF in lung tissue were correlated with the degree of airway remodeling in each group (P 0.001). Conclusion: In rat bronchial asthma airway remodeling model, dexamethasone can effectively inhibit airway remodeling, and early application of dexamethasone can block the progression of airway remodeling, and dexamethasone can down-regulate the expression of IL-8 and EGF. Furthermore, the content of IL-8 EGF was positively correlated with the degree of airway remodeling, which suggested that dexamethasone might interfere with airway remodeling by inhibiting the expression of IL-8 EGF, and IL-8 EGF might also be one of the important indexes to measure the degree of airway remodeling.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R562.25

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