COPD患者外周血中性粒细胞表面PSGL-1、CD44的表达以及香烟烟雾提取物对其表达的影响

发布时间：2018-04-25 10:14

本文选题：慢性阻塞性肺疾病 + 香烟烟雾提取物　；参考：《华中科技大学学报(医学版)》2017年05期

【摘要】：目的探讨P-选择素糖蛋白配体1(P-selectin glycoprotein ligand 1,PSGL-1)和CD44在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者外周血中性粒细胞中的表达,以及香烟烟雾提取物对健康志愿者外周血中性粒细胞PSGL-1和CD44表达的影响。方法收集华中科技大学同济医学院附属同济医院2015年9月至2015年12月呼吸与危重症医学科收治的26例COPD患者(COPD组),以及24例无吸烟史的健康志愿者作为正常对照组(NC组),流式细胞术检测COPD患者和健康志愿者外周血中性粒细胞表面PSGL-1和CD44的表达。同时分离6例健康无吸烟史的志愿者外周血标本中的中性粒细胞,用不同浓度(0%、5%、10%、20%)香烟烟雾提取物(cigarette smoke extract,CSE)刺激,流式细胞术检测中性粒细胞表面PSGL-1和CD44的表达。结果 COPD组患者外周血中性粒细胞表面PSGL-1、CD44的表达较NC组明显增强(均P0.05),CSE刺激的正常人外周血中性粒细胞表面PSGL-1、CD44表达明显增强(均P0.05),且呈浓度依赖性。结论 COPD患者外周血中性粒细胞表面PSGL-1和CD44的表达较正常对照组增强。香烟烟雾提取物可能通过促进外周血中性粒细胞表面PSGL-1和CD44的表达,从而启动中性粒细胞的粘附级联反应,促进气道中性粒细胞炎症反应。
[Abstract]:Objective to investigate the expression of P-selectin glycoprotein ligand 1(P-selectin glycoprotein ligand 1 (PSGL-1) and CD44 in peripheral blood neutrophils from patients with chronic obstructive pulmonary disease (copd). And the effect of cigarette smoke extract on the expression of PSGL-1 and CD44 in peripheral blood neutrophils of healthy volunteers. Methods from September 2015 to December 2015, Tongji Hospital affiliated to Tongji Medical College, Tongji University of Science and Technology, Huazhong University of Science and Technology, 26 patients with COPD and 24 healthy volunteers with no smoking history were collected as normal controls. The expression of PSGL-1 and CD44 on neutrophils in peripheral blood of patients with COPD and healthy volunteers were detected by flow cytometry. At the same time, neutrophils were isolated from 6 healthy volunteers with no smoking history. The neutrophils were stimulated by cigarette smoke extract (smoke) and the expression of PSGL-1 and CD44 on the surface of neutrophils were detected by flow cytometry. Results the expression of PSGL-1 + CD44 on peripheral blood neutrophils in COPD group was significantly higher than that in NC group (P 0.05). Conclusion the expression of PSGL-1 and CD44 on peripheral blood neutrophils in patients with COPD is higher than that in normal controls. Cigarette smoke extract may promote airway neutrophil inflammation by promoting the expression of PSGL-1 and CD44 on the surface of peripheral blood neutrophils, thus initiating the adhesion cascade reaction of neutrophils.
【作者单位】：华中科技大学同济医学院附属同济医院呼吸与危重症医学科;华中科技大学同济医学院基础医学院免疫学系;华中科技大学同济医学院附属同济医院感染科;
【基金】：湖北省自然科学基金资助项目(No.2017CFB765)
【分类号】：R563.9

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