小剂量胰岛素对脓毒症急性肺损伤大鼠肺组织血红素加氧酶-1表达的影响及肺保护作用

发布时间：2018-04-25 20:45

本文选题：胰岛素 + 脓毒症　；参考：《广东医学》2015年24期

【摘要】：目的观察小剂量胰岛素对脓毒症急性肺损伤(ALI)大鼠肺组织血红素加氧酶-1(HO-1)表达的影响,探讨胰岛素的肺保护作用。方法 30只雄性SD大鼠随机分为假手术组、脓毒症组、胰岛素治疗组3组,每组10只。脓毒症组和胰岛素治疗组采用盲肠结扎穿孔法建立脓毒症模型,假手术组开腹后未行盲肠结扎穿孔即关腹;胰岛素治疗组造模后即予皮下注射胰岛素0.5 IU/kg,假手术组和脓毒症组皮下注射等量生理盐水。各组于建模后12 h处死,分别测定血氧分压(PaO_2)、肺湿干质量比、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、肺组织NF-κB/p65含量及HO-1表达量、支气管肺泡灌洗液(BALF)中性粒细胞计数及髓过氧化物酶(MPO)活性,并在光镜下观察肺组织病理表现。结果胰岛素治疗组、脓毒症组PaO_2、SOD、T-AOC均明显低于假手术组(P0.05),而胰岛素治疗组较脓毒症组明显升高(P0.05);胰岛素治疗组、脓毒症组肺湿干质量比明显大于假手术组(P0.05),而胰岛素治疗组较脓毒症组明显降低(P0.05)。胰岛素治疗组、脓毒症组BALF中性粒细胞计数、MPO活性及肺组织NF-κB/p65含量均明显高于假手术组(P0.05),而胰岛素治疗组较脓毒症组明显降低(P0.05)。胰岛素治疗组、脓毒症组肺组织HO-1表达量均明显高于假手术组(P0.05),且胰岛素治疗组较脓毒症组明显升高(P0.05)。脓毒症组肺组织病理学损伤程度高于假手术组,而胰岛素治疗组肺组织炎性病变较脓毒症组明显减轻。结论小剂量胰岛素可能通过抑制NF-κB活化上调HO-1的表达对脓毒症ALI大鼠发挥肺保护作用。
[Abstract]:Objective to observe the effect of low dose insulin on the expression of heme oxygenase-1 (HO-1) in lung tissue of rats with acute lung injury of sepsis and to explore the protective effect of insulin on lung. Methods Thirty male Sprague-Dawley rats were randomly divided into three groups: sham operation group, sepsis group and insulin treatment group. Sepsis model was established by cecal ligation and perforation in sepsis group and insulin treatment group. Insulin treatment group was treated with insulin 0.5 IUP / kg, sham operation group and sepsis group were injected with the same amount of normal saline subcutaneously. The rats were killed at 12 h after modeling. The blood oxygen partial pressure (Pao), lung wet / dry weight ratio, superoxide dismutase (SOD), total antioxidant capacity (T- AOC), the content of NF- 魏 B/p65 and the expression of HO-1 in lung tissue were measured. BALF neutrophil count and myeloperoxidase (MPO) activity in bronchoalveolar lavage fluid (BALF) were observed under light microscope. Results in the insulin treatment group and sepsis group, the P0.05 T-AOC of the Pao _ 2 and SODX were significantly lower than that of the sham operation group, while the insulin treatment group was significantly higher than that of the septic group, and the insulin treatment group was significantly higher than that of the septic group, and the insulin treatment group was significantly higher than that of the septic group. The ratio of wet and dry lung weight in sepsis group was significantly higher than that in sham operation group, while that in insulin treatment group was significantly lower than that in septic group. The BALF neutrophil count and NF- 魏 B/p65 content in insulin treatment group and sepsis group were significantly higher than those in sham operation group (P 0.05), while insulin treatment group was significantly lower than that in sepsis group. The expression of HO-1 in lung tissue of insulin treatment group and sepsis group was significantly higher than that of sham operation group (P 0.05), and that of insulin treatment group was significantly higher than that of sepsis group. The degree of lung histopathological injury in sepsis group was higher than that in sham operation group, while the inflammatory lesion of lung tissue in insulin treatment group was significantly less than that in septic group. Conclusion low dose insulin may play a protective role in the lung of septic ALI rats by inhibiting the activation of NF- 魏 B and up-regulating the expression of HO-1.
【作者单位】：广东省深圳市第三人民医院急诊科;南华大学附属第一医院急诊教研室;
【基金】：湖南省2011年第四批省级科技计划(应用基础研究、科技专项)项目(编号:2011FJ3170)
【分类号】：R459.7;R563

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