不同香烟烟雾暴露时间对大鼠肺组织树突细胞数量的影响研究

发布时间：2018-05-03 13:09

本文选题：树突细胞 + 烟雾　；参考：《中国全科医学》2014年36期

【摘要】：目的探讨不同香烟烟雾暴露时间对大鼠肺组织树突细胞(DCs)数量的影响。方法将清洁级雄性Wistar大鼠50只按照完全随机化法分为空白对照组(A组,10只)和香烟烟雾暴露组(40只),按照不同暴露时间将香烟烟雾暴露组又分为香烟烟雾暴露4周组(B组)、香烟烟雾暴露8周组(C组)、香烟烟雾暴露12周组(D组)和香烟烟雾暴露8周戒烟8周组(E组),每组10只。采用免疫组化法测定大鼠肺组织CD11c和S100蛋白阳性DCs数量的变化。结果 CD11c、S100蛋白阳性DCs均在支气管管壁、炎症区域、血管周围分布明显。5组CD11c、S100蛋白阳性DCs数量比较,差异有统计学意义(F=53.14、27.41,P0.001)。B、C、D、E组CD11c、S100蛋白阳性DCs数量均高于A组,C、D组均高于B组,D组高于C组,E组低于C组(P0.05)。结论香烟烟雾暴露可以导致大鼠肺组织DCs数量增加,且随着香烟烟雾暴露时间的延长,DCs数量增多越明显,戒烟后其数量有所减少。
[Abstract]:Objective to investigate the effects of cigarette smoke exposure time on the number of dendritic cells (DCs) in rat lung tissue. Methods Fifty clean male Wistar rats were randomly divided into control group (n = 10) and cigarette smoke exposure group (n = 40). Cigarette smoke exposure group (n = 40) was divided into cigarette smoke exposure group (n = 4) and cigarette smoke exposure group (n = 40). Group B was exposed to cigarette smoke, group C was exposed to cigarette smoke for 8 weeks, group D was to group D for 12 weeks of cigarette smoke exposure, and group E was given smoking cessation for 8 weeks, with 10 rats in each group. The changes of CD11c and S 100 protein positive DCs in lung tissue of rats were determined by immunohistochemical method. Results the number of CD11cmS100 positive DCs in the bronchial wall, inflammatory area and perivascular distribution was significantly increased in group 5 (P < 0.05). The difference was statistically significant (P 0.001). The number of positive DCs of CD11cfen S100 protein in group A was higher than that in group C (P 0.05) and that in group D was higher than that in group B (P < 0.05). Conclusion cigarette smoke exposure can increase the number of DCs in lung tissue of rats.
【作者单位】：河北省人民医院呼吸二科;北京儿童医院河北儿童医疗国际部;
【分类号】：R563

【参考文献】