甘草酸二胺通过上调水通道蛋白5减轻小鼠急性肺损伤肺水肿的实验研究

发布时间：2018-05-06 14:01

本文选题：甘草酸二胺 + 急性肺损伤　；参考：《四川大学学报(医学版)》2014年03期

【摘要】：目的探讨甘草酸二胺对于急性肺损伤状态下水通道蛋白5(AQP-5)的调节作用及相关机制。方法30只SPF级雄性BALB/c小鼠被随机分为3组,即Control组(对照组)、LPS组(模型组)和LPS+DG组(治疗组),每组10只。通过HE染色观察肺组织病理学改变,并进行肺损伤评分;使用湿/干比(W/D)分析肺水肿程度;RT-PCR和Western blot对AQP-5的表达进行测量;Western blot测定总核因子-κB p65(total NF-κB p65)和磷酸化-NF-κB p65(p-NF-κB p65)蛋白表达。结果小鼠气道内注射LPS 72h后,肺损伤评分和肺组织W/D比值增加,AQP-5表达水平下降,p-NF-κB p65水平增加,差异有统计学意义(P均0.05)。同时发现,LPS+DG组小鼠肺损伤评分、W/D比值和p-NF-κB p65水平较LPS组降低,LPS+DG组AQP-5表达量较LPS组升高,差异均有统计学意义(P均0.05)。结论甘草酸二胺可以有效的减轻LPS诱导的小鼠急性肺损伤(ALI)导致的肺水肿,其机制可能与甘草酸二胺抑制NF-κB p65的活化,上调AQP-5的表达有关。
[Abstract]:Objective to investigate the regulatory effect of diamine glycyrrhizinate (diamine glycyrrhizinate) on aquaporin 5 aquaporin 5 (AQP-5) in acute lung injury. Methods Thirty SPF grade male BALB/c mice were randomly divided into three groups: Control group (model group) and LPS DG group (10 rats in each group). The pathological changes of lung tissue were observed by HE staining, and lung injury score was evaluated by wet / dry ratio (W / D). The expression of total nuclear factor- 魏 B p65(total NF- 魏 B p65) and phosphorylated -NF- 魏 B p65p-NF- 魏 B p65) protein were measured by RT-PCR and Western blot, and the expression of total nuclear factor- 魏 B p65(total NF- 魏 B p65) and phosphorylated -NF- 魏 B p65p-NF- 魏 B p65 protein were measured by Western blot. Results 72 hours after LPS injection, lung injury score and lung WR / D ratio increased, the expression of p-NF- 魏 B p65 increased, and the difference was statistically significant (P 0.05). It was also found that the lung injury score and the level of p-NF- 魏 B p65 in LPS-DG group were significantly lower than those in LPS group. The expression of AQP-5 in LPS-DG group was significantly higher than that in LPS group (P < 0.05). Conclusion diamine glycyrrhizinate can effectively attenuate pulmonary edema induced by LPS induced acute lung injury in mice. The mechanism may be related to the inhibition of NF- 魏 B p65 activation and up-regulation of AQP-5 expression by diamine glycyrrhizinate.
【作者单位】：四川大学华西医院呼吸病学研究室呼吸内科;
【基金】：国家自然科学基金(No.31171103) 四川省应用基础研究项目(No.2013JY0032) 中国博士后科学基金(No.2014M552369)资助
【分类号】：R563

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