藻酸双酯钠对脂多糖所致小鼠肺微血管内皮细胞损伤的保护作用研究

发布时间：2018-05-07 12:18

本文选题：急性肺损伤 + 藻酸双酯钠　；参考：《重庆医学》2016年09期

【摘要】：目的研究藻酸双酯钠(PSS)对脂多糖(LPS)所致的小鼠肺微血管内皮细胞(PMVEC)损伤的保护作用。方法体外培养PMVEC,随机将细胞分为空白对照组(C组)、LPS刺激组(L组)、PSS+LPS组(LP组)。采用MTT法检测PSS对LPS刺激PMVEC的细胞活力的影响,虎红染色法测定中性粒细胞(PMN)对PMVEC黏附数量的影响,酶联免疫吸附试验(ELISA)法检测3组培养上清液中肿瘤坏死因子-α(TNF-α)和细胞间黏附分子-1(ICAM-1)的浓度。结果 PSS能抑制LPS所致的PMVEC细胞活力下降(P=0.001);与C组比较,L组的PMVEC与PMN的黏附数量明显增加(P=0.000),TNF-α和ICAM-1的表达显著增加(P=0.000);与L组比较,PSS预处理1h能显著降低LPS所致的PMVEC与PMN的黏附(P=0.000),LP组的TNF-α和ICAM-1表达显著降低(P0.05)。结论 PSS能抑制LPS对PMVEC的损伤及PMVEC与PMN的黏附,其作用机制可能与降低ICAM-1和TNF-α的表达有关。
[Abstract]:Objective to study the protective effect of sodium alginate (PSS) on PMVECs induced by lipopolysaccharide (LPS) in mice pulmonary microvascular endothelial cells (PMVECs). Methods PMVECs were cultured in vitro. The cells were randomly divided into two groups: control group (C group), LPS-stimulated group (L group) and PSS LPS group (LP group). MTT assay was used to detect the effect of PSS on the cell viability of PMVEC stimulated by LPS, and the effect of neutrophil PMNs on the adhesion of PMVEC was determined by tiger red staining. The concentrations of tumor necrosis factor- 伪 (TNF- 伪) and intercellular adhesion molecule-1 (ICAM-1) in the supernatants of the three groups were detected by Elisa. Results PSS could inhibit the decrease of PMVEC cell viability induced by LPS, and the adhesion between PMVEC and PMN in group L was significantly increased compared with group C, and the expression of TNF- 伪 and ICAM-1 was significantly increased in group L, and the PMVEC induced by LPS was significantly decreased after 1 h of pretreatment with PSS compared with group L. The expression of TNF- 伪 and ICAM-1 in LP group was significantly lower than that in P0. 05 group. Conclusion PSS can inhibit the damage of LPS to PMVEC and the adhesion of PMVEC to PMN, and its mechanism may be related to the decrease of ICAM-1 and TNF- 伪 expression.
【作者单位】：重庆医科大学附属永川医院麻醉科;四川省革命伤残军人医院麻醉科;
【基金】：重庆医科大学附属永川医院青年课题(YJQN201002)
【分类号】：R563

【相似文献】