沙丁胺醇对COPD兔支气管插管单肺通气中LTB4浓度的影响

发布时间：2018-05-10 07:35

本文选题：肺疾病 + 慢性阻塞性　；参考：《重庆医学》2017年03期

【摘要】：目的观察沙丁胺醇对烟熏致慢性阻塞性肺疾病(COPD)实验兔模型单肺通气后血清和肺泡灌洗液中白三烯B4(LTB4)浓度的影响。方法健康新西兰大白兔18只分为正常组、COPD组、COPD+沙丁胺醇组,每组6只。除正常组以外的其他两组均采用实验组自行创造的方法构建了单纯烟熏致COPD实验兔模型。分别在给实验兔行右主支气管插管前(T0),插管后即刻(T1),插管后行单肺通气30min(T2),插管后行单肺通气60min(T3)采血测LTB4,行右主支气管插管单肺通气60min后处死实验兔,解剖后取少量双肺上叶作组织病理学观察,取适量支气管肺泡灌洗液测LTB4的浓度。结果 COPD组中实验兔支气管肺泡灌洗液中LTB4的浓度比正常组明显升高(P0.05),COPD+沙丁胺醇组中LTB4的浓度比COPD组明显降低(P0.05)。肺组织形态学检查显示,COPD组观察到炎性细胞侵入,肺组织有明显的炎症性改变;COPD+沙丁胺醇组可见炎性细胞明显减少,肺组织炎症性改变有一定改善。结论支气管插管单肺通气中吸入沙丁胺醇能抑制LTB4的浓度。
[Abstract]:Objective to observe the effect of salbutamol on the concentration of leukotriene B _ 4 and LTB _ 4 in serum and alveolar lavage fluid after single lung ventilation in fumigated chronic obstructive pulmonary disease (COPD) rabbits. Methods 18 healthy New Zealand rabbits were divided into normal group and COPD group with 6 in each group. In addition to the normal group, all the other two groups established the COPD experimental rabbit model by the method of self-created by the experimental group. The blood samples were collected before right main bronchus intubation, immediately after intubation, 30 minutes after intubation and 60 minutes after intubation. LTB4 was measured. The rabbits were killed after right main bronchus intubation with single lung ventilation (60min). After dissection, a small amount of upper lobes of lung were taken for histopathological observation, and appropriate amount of bronchoalveolar lavage fluid (BALF) was taken to measure the concentration of LTB4. Results the concentration of LTB4 in bronchoalveolar lavage fluid in COPD group was significantly higher than that in normal group. The concentration of LTB4 in COPD group was significantly lower than that in COPD group. Pulmonary histomorphologic examination showed that inflammatory cell invasion was observed in COPD group, and there were obvious inflammatory changes in lung tissue. In COPD salbutamol group, inflammatory cells were significantly decreased, and inflammatory changes in lung tissue were improved to some extent. Conclusion inhalation of salbutamol can inhibit the concentration of LTB4 during single lung ventilation.
【作者单位】：河北北方学院附属第一医院麻醉科;河北省张家口市沙岭子医院;河北北方学院第一临床学院;
【分类号】：R563.9

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