脂多糖诱导急性肺损伤小鼠肺脏蛋白质电泳图谱分析

发布时间：2018-05-11 05:58

本文选题：急性肺损伤 + 脂多糖　；参考：《安徽医科大学学报》2017年05期

【摘要】：目的蛋白质双向电泳技术观察气管滴注脂多糖诱导急性肺损伤后小鼠肺组织在蛋白质水平上的改变。方法小鼠气管内滴注脂多糖制备小鼠急性肺损伤模型,48 h后做小鼠肺CT、肺病理切片及测肺湿/干重比用于观察肺损伤程度;并提取总蛋白,进行双向电泳测定,对获取的蛋白图谱进行分析,寻找差异表达的蛋白质。结果实验组肺CT显示明显炎症表现;病理切片显示小鼠肺组织水肿充血改变明显,肺泡中有大量红细胞和中性粒细胞浸润,肺损伤评分和肺湿/干重比正常组增加;双向电泳结果显示实验组小鼠比正常组小鼠的肺组织总蛋白数增加,蛋白质点数为(100±2),其中有21个表达的蛋白差异点,通过蛋白质谱鉴定和生物信息检索结果显示蛋白质过氧化物酶6在实验组和正常组中有明显差异,其可能与急性肺损伤的发生相关。结论通过向小鼠气管内滴注脂多糖可以诱发急性肺损伤,蛋白质双向电泳结果显示实验组小鼠肺组织中蛋白质过氧化物酶6表达的增加,机制还待进一步研究。
[Abstract]:Objective to observe the changes of protein level in lung tissue of mice after acute lung injury induced by endotracheal instillation of lipopolysaccharide (LPS). Methods the acute lung injury model of mice was induced by intratracheal instillation of lipopolysaccharide (LPS) for 48 hours. Lung biopsy and lung wet / dry weight ratio were used to observe the severity of lung injury, and total protein was extracted and determined by two-dimensional electrophoresis. The obtained protein maps were analyzed to find differentially expressed proteins. Results the lung CT showed obvious inflammatory manifestations in the experimental group, and the pathological sections showed that the edema and congestion of lung tissue were obvious in the experimental group, a large number of red blood cells and neutrophils infiltrated in the alveoli, and the lung injury score and lung wet / dry weight increased compared with the normal group. The results of two dimensional electrophoresis showed that the number of total protein in lung tissue of the experimental group was higher than that of the normal group, and the protein number was 100 卤2, among which there were 21 differentially expressed protein points. The results of protein spectrum identification and bioinformatics retrieval showed that protein peroxidase 6 was significantly different between the experimental group and the normal group, which might be related to the occurrence of acute lung injury. Conclusion Acute lung injury can be induced by intratracheal infusion of lipopolysaccharide in mice. The results of two-dimensional protein electrophoresis show that the expression of protein peroxidase 6 in lung tissue of experimental group is increased, and the mechanism needs further study.
【作者单位】：安徽医科大学解放军307医院临床学院;军事医学科学院附属医院呼吸与危重症医学科;
【基金】：国家科技重大专项(编号:2013ZX10004203) 解放军307医院创新科研基金(编号:FC-2014-01)
【分类号】：R563.8

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