5-羟色胺转运体基因多态性与慢性阻塞性肺疾病相关性研究

发布时间：2018-05-13 20:53

本文选题：-羟色胺 + -羟色胺转运体　；参考：《中国药理学通报》2017年05期

【摘要】：目的探讨5-羟色胺转运体(SERT)基因多态性与慢性阻塞性肺疾病(COPD)的相关性。方法按入选、排除标准,选择COPD患者51例,对照组为同期健康体检者49例。用PCR方法检测SERT基因多态性;用Real-time RT-PCR方法检测SERT基因mRNA表达。对COPD组患者进行肺功能检查,记录第1秒用力呼气量(FEV1)、用力肺活量(FVC)和FEV1/FVC%。结果无论COPD组还是健康对照组,均发现3种SERT基因型L/L(528/528)、L/S(528/484)和S/S(484/484),其中SS基因型为主要基因型;COPD组SERT基因表达明显高于对照组;COPD组伴有肺动脉高压(PAH)患者的L等位基因频率明显高于对照组;COPD组LS基因型患者COPD发病年龄小于SS基因型患者。结论 SERT基因多态性与COPD的遗传易感性相关,对促进COPD形成及诱发PAH有一定作用。
[Abstract]:Objective to investigate the association between serotonin transporter (serotonin) gene polymorphism and chronic obstructive pulmonary disease (COPD). Methods 51 COPD patients were selected according to the inclusion and exclusion criteria, and 49 healthy persons in the control group. The polymorphism of SERT gene was detected by PCR and mRNA expression of SERT gene was detected by Real-time RT-PCR method. Pulmonary function was measured in COPD group. Forced expiratory volume (FEV1), forced vital capacity (FVC) and FEV1 / FVC were recorded in the first second. Results No matter the COPD group or the healthy control group, Three SERT genotypes, L / L 528 / 528 / 484) and S / S 484 / 484, were found. The L allele frequency of SS genotype was significantly higher than that of control group with pulmonary hypertension. The age of onset of COPD in type A patients was lower than that in patients with SS genotype. Conclusion the polymorphism of SERT gene is associated with the genetic susceptibility of COPD, which plays a role in promoting the formation of COPD and inducing PAH.
【作者单位】：中山市人民医院药学部;中山市人民医院呼吸内科;中国医科大学临床药理学教研室;
【基金】：国家自然科学基金资助项目(No 81273511) 广东省医学科研基金资助项目(No A2010744)
【分类号】：R563.9

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