17β-雌二醇对小鼠肺成纤维细胞细胞周期的影响

发布时间：2018-05-14 00:31

  本文选题：肺成纤维细胞 + β-雌二醇　； 参考：《郑州大学学报(医学版)》2014年04期

【摘要】：目的:观察17β-雌二醇(17β-E2)对小鼠肺成纤维细胞细胞周期的影响。方法:根据L9(33)正交实验设计,用不同质量浓度SiO2(20、50、100 mg/L)诱导小鼠肺成纤维细胞,再用不同浓度17β-E2(10-6、10-7、10-8mol/L)干预不同时间(24、48、72 h),MTT法检测小鼠肺成纤维细胞的增殖状况,以增殖活性较低为筛选标准,选取最佳实验条件。将小鼠肺成纤维细胞分成3组:空白对照组、SiO2诱导组和17β-E2干预组,在相应实验条件下培养细胞,运用流式细胞术检测细胞周期。结果:最佳实验条件筛选结果为:用20 mg/L SiO2诱导,用10-6mol/L 17β-E2干预24 h。3组G1期细胞比例、S+G2期细胞比例差异均有统计学意义(F=50.300、29.896,P0.001)。SiO2诱导组G1期细胞比例较空白对照组降低,S+G2期细胞比例升高;而17β-E2干预组G1期细胞比例较SiO2诱导组升高,S+G2期细胞比例降低(P0.05)。结论:17β-E2可能是通过阻滞小鼠肺成纤维细胞由G1期进入S期和G2期,从而抑制其增殖。
[Abstract]:Aim: to observe the effect of 17 尾 -estradiol 17 尾 -E _ 2 on the cell cycle of mouse lung fibroblasts. Methods: mouse lung fibroblasts were induced by different concentrations of SiO2O2050mg / L) according to the L9 / 33) orthogonal design. The proliferation of mouse lung fibroblasts was detected by MTT assay with different concentrations of 17 尾 -E2O10-6 (10-7) 10-8 mol / L at different time points. The optimum experimental conditions were selected with low proliferative activity as the screening criterion. The mouse lung fibroblasts were divided into three groups: the blank control group induced by SiO2 and the 17 尾 -E2 intervention group. The cells were cultured under the corresponding experimental conditions, and the cell cycle was detected by flow cytometry. Results: the optimal experimental conditions were as follows: induced by 20 mg/L SiO2, The ratio of G 1 phase cells and S G 2 phase cells in 10-6mol/L 17 尾 -E2 group were significantly different from those in the control group. The ratio of G 1 phase cells in the G 1 phase group induced by 10-6mol/L 17 尾 -E2 was significantly lower than that in the control group, and the percentage of S G 2 phase cells in the S G 2 phase group was significantly higher than that in the control group. The proportion of G 1 phase cells in 17 尾 -E2 treated group was higher than that in SiO2 induced group, and the proportion of S G 2 phase cells was decreased (P 0.05). Conclusion the proliferation of mouse lung fibroblasts may be inhibited by blocking the proliferation of mouse lung fibroblasts from G _ 1 to S and G _ 2.
【作者单位】： 河北联合大学公共卫生学院流行病与卫生统计学教研室;
【基金】：河北省科学技术研究与发展计划项目10276113D 河北省教育厅课题2006139
【分类号】：R135.2
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本文编号：1885535