动态观察过敏原诱导的哮喘小鼠模型从急性炎症到慢性重塑的发展

发布时间：2018-05-14 23:18

本文选题：过敏原 + 气道炎症　；参考：《中国病理生理杂志》2017年02期

【摘要】：目的:动态观察对比卵白蛋白(OVA)诱导的哮喘小鼠模型从气道的急性炎症到慢性重塑的相关炎症指标及气道重塑指标的变化。方法:60只雌性BALB/c小鼠随机分为正常对照组和哮喘组。哮喘组给予OVA致敏和激发,正常对照组给予等量生理盐水(NS)作为对照,第21天开始,连续激发3 d,观察急性炎症反应;而后每周激发1次、每次激发后24 h内取材1次连续5周;动态观察对比OVA激发组和NS对照组小鼠气道炎症细胞的浸润情况、灌洗液细胞因子水平和气道重塑相关指标。结果:与NS组比较,OVA组肺组织的炎症细胞浸润明显,炎症因子升高;气道上皮细胞向杯状细胞转化明显;支气管外周胶原沉积显著。OVA组内比较,24 d组炎症细胞和炎症因子上升明显,而上皮细胞向杯状细胞的转化以及支气管外周胶原沉积相关重塑指标不明显,52 d组上皮细胞向杯状细胞的转化以及支气管外周胶原沉积相关重塑指标最为显著,而炎症细胞和炎症因子有所下降。结论:连续3次OVA激发可成功模拟炎症细胞和炎症因子显著的急性哮喘模型;每周1次、连续5周的OVA激发方式可成功模拟重塑明显的慢性哮喘模型。
[Abstract]:Aim: to observe and compare the changes of airway remodeling indexes and inflammatory indexes from acute airway inflammation to chronic remodeling in asthmatic mice induced by ovalbumin (OVA). Methods 60 female BALB/c mice were randomly divided into normal control group and asthma group. The asthmatic group was sensitized and stimulated with OVA, while the normal control group was treated with the same amount of normal saline as control group. From the 21st day on, the acute inflammatory reaction was observed for 3 days, then the acute inflammatory reaction was observed once a week. The samples were collected for 5 weeks within 24 hours after each stimulation. The infiltration of airway inflammatory cells, the level of cytokines in the lavage fluid and the relative indexes of airway remodeling were observed and compared between the OVA challenge group and the NS control group. Results: compared with NS group, the inflammatory cells in the lung tissue of OVA group were obviously infiltrated, the inflammatory factors were increased, and the airway epithelial cells were transformed into goblet cells. Bronchial peripheral collagen deposition was significant. In OVA group, inflammatory cells and inflammatory factors increased significantly in 24 d group. However, the transformation of epithelial cells to goblet cells and the remodeling indexes related to the deposition of collagen on the periphery of the bronchus were not obvious in the 52 d group, and the transformation from the epithelial cells to the goblet cells was the most significant in the group of 52 days. Inflammatory cells and inflammatory factors decreased. Conclusion: the model of acute asthma with significant inflammatory cells and inflammatory factors can be successfully simulated by OVA stimulation for three consecutive times, and the model of chronic asthma with obvious remodeling can be successfully simulated by OVA stimulation once a week for 5 weeks.
【作者单位】：第四军医大学西京医院呼吸内科;
【基金】：国家自然科学基金资助项目(No.81070028)
【分类号】：R562.25;R-332

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