rHSG对COPD大鼠小气道阻力及成纤维细胞TGF-β1表达的影响

发布时间：2018-05-15 23:18

本文选题：慢性阻塞性肺疾病 + 小气道阻力　；参考：《基础医学与临床》2014年11期

【摘要】：目的检测正常COPD大鼠小气道成纤维细胞中r HSG的表达,寻找其与小气道阻力、TGF-β1的相关性,探索r HSG调控气道重塑的机制。方法将大鼠随机分为对照组及模型组,分别检测肺功能和肺泡灌洗液中TGF-β1含量。用组织块贴壁法培养气道成纤维细胞,用RT-PCR和Western blot检测r HSG mRNA和蛋白的表达。分析r HSG基因量与小气道阻力以及TGF-β1的相关性。结果模型组大鼠先后出现咳嗽、气急、喘鸣和痰多等症状并伴有精神萎靡、纳少和毛发枯黄等,之后出现体质量减轻,肺体积略增大,肺顺应性明显下降,而气道阻力明显高于对照组(P0.01);肺泡灌洗液中TGF-β1含量均明显高于对照组(P0.01);r HSG基因在对照组中表达高于模型组(P0.05);r HSG mRNA和蛋白与气道阻力(RI)、TGF-β1呈负相关,与肺顺应性(cydn)呈正相关。结论 r HSG可能通过调控TGF-β1水平干预气道重塑过程。
[Abstract]:Objective to detect the expression of r HSG in small airway fibroblasts of normal COPD rats, to find out the correlation between r HSG and TGF- 尾 1, and to explore the mechanism of r HSG regulating airway remodeling. Methods Rats were randomly divided into control group and model group. Lung function and TGF- 尾 1 content in alveolar lavage fluid were measured. Airway fibroblasts were cultured by tissue mass adherence, and the expression of r HSG mRNA and protein were detected by RT-PCR and Western blot. The correlation between r HSG gene and small airway resistance and TGF- 尾 1 was analyzed. Results in the model group, symptoms such as cough, shortness of breath, wheezing and phlegm were found successively, accompanied by mental depression, low intake and yellow hair, and then the body mass decreased, the lung volume increased slightly, and the lung compliance decreased significantly. The level of TGF- 尾 1 in alveolar lavage fluid was significantly higher than that in control group, and the expression of TGF- 尾 1 in alveolar lavage fluid was significantly higher than that in model group. The expression of TGF- 尾 1 in alveolar lavage fluid was negatively correlated with TGF- 尾 1 and positively correlated with lung compliance. Conclusion r HSG may interfere with airway remodeling by regulating TGF- 尾 1 level.
【作者单位】：贵阳中医学院第二附属医院呼吸内科;
【基金】：国家自然科学基金(81060319) 贵州省科学技术基金[黔科合基字(2008I2177号)]
【分类号】：R563.9

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