抗血管内皮细胞抗体在大鼠肺气肿中的作用及其与甲强龙干预的关系研究

发布时间：2018-05-19 15:10

本文选题：肺气肿 + 吸烟　；参考：《中国生化药物杂志》2014年04期

【摘要】：目的观察抗血管内皮细胞抗体(anti-endothelial cells antibodies,AECA)在吸烟诱导的肺气肿中的作用,以及甲强龙的干预影响。方法 39只大鼠随机分为正常组、模型组及甲强龙干预组(n=13)。模型组及干预组进行被动吸烟,烟雾暴露1个月时,对干预组皮下注射甲强龙(10 mg/kg,1次/d),每周6 d;烟雾暴露90 d后,抽取下腔静脉血,处死大鼠,同时收集支气管肺泡灌洗液(bronchial alveolar lavage fluid,BALF);检测各组大鼠血清、支气管肺泡灌洗液中的AECA。肺组织切片苏木素伊红染色后观察组织形态学改变,测定肺平均内村间隔(mean linear intercept,MLI)、平均肺泡数(mean alveolar numbers,MAN)。结果与正常组、干预组比较,模型组血清及支气管肺泡灌洗液中的AECA、MLI升高(P0.05),但MAN显著降低(P0.05);肺泡灌洗液AECA与MLI正相关(r=0.821,P0.05),与MAN负相关(r=-0.894,P0.05)。结论 AECA可能参与吸烟诱导的肺气肿形成,并可能与其严重程度相关;甲强龙可能通过降低AECA含量影响肺气肿形成。
[Abstract]:Objective to investigate the role of anti-endothelial cells antibodies-AECA in smoking induced emphysema and the effect of methylenolone on emphysema. Methods Thirty-nine rats were randomly divided into three groups: normal group, model group and methylprednisolone intervention group. The model group and the intervention group were given passive smoking. After one month of smoke exposure, the rats in the intervention group were subcutaneously injected with 10 mg 路kg ~ (-1) 路kg ~ (-1) of metopropanolone for 6 days a week. After 90 days of smoke exposure, the rats were killed by taking blood from the inferior vena cava. At the same time, bronchial alveolar lavage fluididine was collected and AECA in serum and bronchoalveolar lavage fluid of rats in each group were detected. The histomorphologic changes were observed after hematoxylin eosin staining in lung sections. The mean linear interceptance (MLI) and mean alveolar number (MAN) were measured. Results compared with the normal group and the intervention group, the serum and bronchoalveolar lavage fluid (BALF) of the model group were significantly higher than those of the control group (P 0.05), but MAN was significantly lower than that of the control group (P 0.05), and the AECA in the alveolar lavage fluid was positively correlated with MLI (0.821 P 0.05), and negatively correlated with MAN (r 0. 894 P 0 05). Conclusion AECA may be involved in the formation of emphysema induced by smoking and may be related to the severity of emphysema, and methylenolone may influence the formation of emphysema by reducing the content of AECA.
【作者单位】：贵州省人民医院呼吸与危重症医学科;
【基金】：国家自然科学基金(81060006) 贵州省科学技术基金黔科合J字[2011]2251 十二五国家科技支撑计划课题(2013BAI09B09)
【分类号】：R563.9

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