干扰素调节因子4在过敏性哮喘发病中的作用

发布时间：2018-05-22 12:35

本文选题：过敏性哮喘 + 干扰素调节因子4　；参考：《武汉大学》2012年博士论文

【摘要】：目的：支气管哮喘是由多种细胞参与的慢性气道炎症性疾病。研究表明,过敏性哮喘受辅助性T细胞的调节,而干扰素调节因子4在辅助性T细胞的分化中起着很重要的作用。因此,本研究探讨干扰素调节因子4是否在过敏性哮喘病人的全身免疫反应中起着一定的作用。方法：分别收集29个过敏性哮喘患者(过敏性哮喘组)和12个健康志愿者(正常对照组)的外周血15ml,采用密度梯度离心法分离出外周血单个核细胞。用实时定量逆转录PCR法和western blot法检测外周血单个核细胞中干扰素调节因子4的基因和蛋白表达含量。用流式细胞仪检测外周血单个核细胞中辅助性T细胞1、辅助性T细胞2和辅助性T细胞17的比例。用ELISA法检测血浆和细胞培养上清中的辅助性T细胞1、辅助性T细胞2和辅助性T细胞17的代表性因子干扰素γ(IFN-γ)、白介素4(IL-4)和白介素17(IL-17)的含量。用实时定量逆转录PCR法检测分别控制辅助性T细胞2、辅助性T细胞17和调节性T细胞分化的转录因子GATA3γt和FOXP3。结果：干扰素调节因子4在过敏性哮喘组中的表达较正常对照组明显增多。辅助性T细胞2和辅助性T细胞17及它们的代表性因子IL-4和IL-17在过敏性哮喘组中的数量较正常对照组明显增多。辅助性T细胞1及其代表性因子IFN-γ生过敏性哮喘组和正常对照组中的表达没有明显变化。过敏性哮喘组干扰素调节因子4的表达分别与辅助性T细胞2、辅助性T细胞17和调节性T细胞的关键性转录因子GATA3、RORγt和FOXP3呈正相关。结论：我们的研究结果提示,干扰素调节因子4可能在转录因子水平促进过敏性哮喘患者辅助性T细胞2和辅助性T细胞17的分化,在过敏性哮喘的发生发展中起一定的作用。
[Abstract]:Objective: bronchial asthma is a chronic airway inflammatory disease involving many kinds of cells. Studies have shown that allergic asthma is regulated by helper T cells, while interferon regulatory factor 4 plays an important role in the differentiation of helper T cells. Therefore, this study investigated whether interferon regulatory factor 4 may play a role in systemic immune response in allergic asthma patients. Methods: peripheral blood mononuclear cells were isolated from 29 allergic asthma patients (allergic asthma group) and 12 healthy volunteers (normal control group) by density gradient centrifugation. The gene and protein expression of interferon regulatory factor 4 in peripheral blood mononuclear cells (PBMC) was detected by real-time quantitative reverse transcription PCR and western blot. The proportion of helper T cell 1, helper T cell 2 and helper T cell 17 in peripheral blood mononuclear cells (PBMC) was determined by flow cytometry. The levels of helper T cells (1), helper T cells (2) and IFN- 纬 (IL-4) and IL-17 (IL-17) in plasma and cell culture supernatant were determined by ELISA method. The transcription factors GATA3 纬 t and FOXP3, which control helper T cell 2, helper T cell 17 and regulatory T cell differentiation, were detected by real-time quantitative reverse transcription PCR. Results: the expression of interferon regulatory factor 4 in allergic asthma group was significantly higher than that in normal control group. The number of helper T cell 2 and helper T cell 17 and their representative factors IL-4 and IL-17 in allergic asthma group were significantly higher than that in normal control group. The expression of helper T cell 1 and its representative factor IFN- 纬 in allergic asthma group and normal control group did not change significantly. In allergic asthma group, the expression of interferon regulatory factor 4 was positively correlated with helper T cell 2, helper T cell 17 and regulatory T cell critical transcription factor GATA3, ROR 纬 t and FOXP3, respectively. Conclusion: our results suggest that interferon regulatory factor 4 may promote the differentiation of helper T cell 2 and helper T cell 17 in allergic asthma patients at the transcription factor level, and may play a role in the occurrence and development of allergic asthma.
【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R562.25

【相似文献】