TWEAK在COPD大鼠股四头肌萎缩中的作用机制研究

发布时间：2018-05-22 19:07

本文选题：慢性阻塞性肺疾病 + 股四头肌萎缩　；参考：《中南大学》2013年硕士论文

【摘要】：目的：TWEAK在COPD大鼠股四头肌萎缩中的作用机制及可能的信号转导通路。方法：8周龄50只Wistar大鼠,按随机法分对照组20只、COPD模型组30只,COPD模型组采用烟熏+气管内滴注猪胰弹性蛋白酶方法建立COPD模型(共三个月)；模型评估：COPD模型组以HE染色观察肺和气道组织病理变化、肺功能及大鼠表现判断造模是否成功。分别使用免疫组化法及Western blot法测定股四头肌中NF-κB、 MuRF1和TWEAK表达并分析其相关性；所有数据使用SPSS18.0软件进行分析。结果： 1.COPD模型组大鼠造模成功,肺功能测定及肺组织病理结果显示均符合COPD诊断标准。 2.COPD模型组大鼠体重以及一侧股四头肌的重量较正常对照组均有明显减轻(P0.05,P0.05)。 3.COPD模型组大鼠股四头肌中TWEAK的表达明显高于正常对照组大鼠(P0.05)。 4. COPD模型组大鼠股四头肌中NF-κB以及MuRF1的表达明显高于对照组大鼠(P0.05,P0.05) 5.各组大鼠股四头肌NF-κB、MuRF1的表达与TWEAK的表达相关性分析呈正相关(P0.05,P0.05) 6.各组大鼠股四头肌中MuRF1与NF-κB的表达相关性分析呈正相关(P0.05)。结论： 1.COPD模型组大鼠股四头肌中TWEAK表达明显增加,且与大鼠股四头肌萎缩有着密切的关系。 2.泛素-蛋白酶体途径可能参与COPD大鼠股四头肌萎缩。 3. TWEAK引起COPD大鼠股四头肌萎缩可能与泛素-蛋白酶体途径的激活相关,且NF-κB可能是其中一个重要的调节因子。含图22幅,表11个,参考文献46篇。
[Abstract]:Objective to investigate the mechanism and the possible signal transduction pathway of TWEAK on quadriceps femoris atrophy in COPD rats. Methods 50 Wistar rats at the age of 8 weeks, The COPD model was established by injecting porcine pancreatic elastase into smoke and trachea (3 months), and the pathological changes of lung and airway tissues were observed by HE staining in the model group. Lung function and rat performance were used to determine whether the model was successful or not. The expressions of NF- 魏 B, MuRF1 and TWEAK in quadriceps femoris were detected by immunohistochemical method and Western blot method, respectively. All the data were analyzed by SPSS18.0 software. Results: In 1.COPD model group, the model was successfully established, and the pulmonary function test and pathological results of lung tissue were in accordance with the diagnostic criteria of COPD. The body weight and the weight of quadriceps femoris in 2.COPD group were significantly decreased compared with the control group. The expression of TWEAK in quadriceps femoris in 3.COPD group was significantly higher than that in normal control group (P 0.05). 4. The expression of NF- 魏 B and MuRF1 in quadriceps femoris in COPD group was significantly higher than that in control group. 5. Correlation analysis between NF- 魏 BnMuRF1 expression and TWEAK expression in quadriceps femoris of rats in each group 6. Correlation analysis of MuRF1 and NF- 魏 B expression in quadriceps femoris of rats in each group (P 0.05). Conclusion: The expression of TWEAK in quadriceps femoris in 1.COPD model group was significantly increased and was closely related to quadriceps femoris atrophy. 2. The ubiquitin-proteasome pathway may be involved in quadriceps femoris atrophy in COPD rats. 3. TWEAK induced quadriceps femoris atrophy in COPD rats may be related to the activation of ubiquitin proteasome pathway, and NF- 魏 B may be one of the important regulatory factors. There are 22 figures, 11 tables and 46 references.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.9

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