RAGE与腹腔注射脂多糖诱导新生大鼠急性肺损伤的相关性研究

发布时间：2018-05-23 20:39

本文选题：脂多糖 + 晚期糖基化终末产物受体　；参考：《南京医科大学学报(自然科学版)》2014年08期

【摘要】：目的:通过腹腔注射途径建立脂多糖(lipopolysaccharide,LPS)诱导的新生大鼠急性肺损伤(acute lung injury,ALI)模型,探讨其与肺组织晚期糖基化终末产物受体(receptor for advanced glycation end-products,RAGE)的相关性。方法:30只SD新生大鼠随机分为5组,每组6只,根据注射LPS剂量分为0.3、1.0、3.0、9.0 mg/kg组及生理盐水对照组,24 h后观察大鼠的一般情况;ELISA法检测血清及支气管肺泡灌洗液(BALF)中肿瘤坏死因子-α(TNF-α)、可溶性RAGE(sRAGE)含量;Western blot法检测肺组织RAGE蛋白水平;real time-PCR法检测RAGE mRNA表达水平;HE染色下对肺组织进行病理学评估。结果:LPS 9mg/kg组死亡率高达83.3%,故未计入实验组;其余4组结果显示,TNF-α水平、肺组织RAGE蛋白、mRNA及肺组织损伤评分随梯度剂量的增加而升高,而BALF中sRAGE水平随剂量增加而降低。结论:腹腔注射LPS可以诱导产生新生大鼠的ALI,LPS剂量为3 mg/kg可作为模型最佳诱导剂量;RAGE可作为ALI的敏感指标。
[Abstract]:Aim: to establish a lipopolysaccharide (LPS) -induced acute lung injury-induced Ali model in neonatal rats by intraperitoneal injection, and to investigate its correlation with advanced glycosylation end product receptor for advanced glycation end-products raga in lung tissue. Methods Thirty Sprague-Dawley rats were randomly divided into 5 groups with 6 rats in each group. General conditions of 24 hours after injection of LPS were divided into 0. 3 and 1. 0 ~ 3. 0 mg/kg group and normal saline control group for 24 hours, the serum and bronchoalveolar lavage fluid (bronchoalveolar lavage fluid) were used to detect tumor necrosis factor- 伪 (TNF- 伪) and soluble RAGEN RAGES. Western blot method was used to detect lung tissue. The expression of RAGE mRNA was detected by RAGE protein level and real time-PCR method. The pathological evaluation of lung tissue was carried out under HE staining. Results the mortality rate of the 9mg/kg group was 83.3%, so it was not included in the experimental group. The results of the other four groups showed that the level of TNF- 伪, the lung tissue RAGE protein mRNA and the lung injury score increased with the increase of the gradient dose, while the sRAGE level in BALF decreased with the increase of the dose. Conclusion: intraperitoneal injection of LPS can induce neonatal rats. The dose of lipopolysaccharide is 3 mg/kg, which can be used as a sensitive index of ALI.
【作者单位】：南京医科大学附属淮安第一医院新生儿科;
【基金】：淮安市科技计划项目(HASZ2012014)
【分类号】：R563.8

【共引文献】