320排CT容积灌注对百草枯致化学性肺损伤的早期评价

发布时间：2018-05-25 19:38

本文选题：计算机断层扫描 + 百草枯　；参考：《河北北方学院》2013年硕士论文

【摘要】：建立合适的百草枯致肺损伤的模型,为后续研究提供必要的条件。健康新西兰大白兔70只,随机分为四组,每小组10只,分别以不同剂量百草枯染毒,第一小组百草枯剂量25mg.kg-1、第二小组剂35mg.kg-1、第三小组剂量45mg.kg-1第四小组剂量50mg.kg-l,确定半数致死量。分别在预设时间点1h、6h、12h、24h、48h及72h每组抽取3只,开胸取双肺上、中、下野6处标本,分别进行(1)HE染色检查；(2)电镜超微结构观察。以百草枯35mg.kg-1剂量选为最适剂量,做为后续CT灌注对化学性肺损伤的稳定、可靠的模型。研究320排CT容积灌注对化学性肺损伤的早期血流动力学改变评价,且与病理学对照,以寻求CT灌注(CT Perfusion)在化学性肺损伤早期功能学改变的监测及诊断价值。30只新西兰大白兔分为对照组及实验组(0时刻为对照组)。采用百草枯(paraquat,PQ)35mg.kg剂量为最适剂量,于Oh、2h、6h、12h、24h、48h行320排胸部CT灌注扫描,并于上述时间点空气栓塞处死4只取肺组织做病理检测。(1)对照组肺的rBF、rBV及rPS平均值分别为128.54士31.31、25.20士4.15、13.06士3.05；(2)实验组肺CTP参数变化与病理改变有显著相关性。毛细血管内皮细胞肿胀程度评级和血流量呈负相关,r=-0.848,p=0.033。毛细血管基底膜破坏程度评级和血管通透性呈正相关,r=0.893,p=0.016。百草枯投药后,随时间的推移肺血流量呈逐渐下降趋势,方差分析提示24小时血流量较未给药时(0时刻)有显著下降,p0.001。百草枯投药后,随时间的推移肺血管通透性呈逐渐上升趋势,方差分析提示2小时血管通透性较未给药时(0时刻)有显著变化,p0.001。血浆TGF-β1含量变化与灌注参数：PS的相关性检验结果:Pearson相关系r=0.07580,p=0.68520.05,血浆TNF-a含量变化与灌注参数PS的相关性检验结果:Pearson相关系r=0.42081,P=0.385620.05。均无统计学差异,说明血浆TGF-β1、TNF-a变化与灌注参数PS无显著相关性。CTP参数(尤其是PS)能反映百草枯所致化学性肺损伤超早期血流动力学异常改变,有望用于早期预报及动态监测早期肺损伤的发生。
[Abstract]:A suitable model of paraquat induced lung injury was established to provide necessary conditions for further study. 70 healthy New Zealand white rabbits were randomly divided into four groups, 10 rabbits in each group. The first group was treated with paraquat 25 mg 路kg -1, the second group 35 mg 路kg -1, the third group 45mg.kg-1 group 4 50 mg 路kg -1, and the second group 50 mg 路kg -1. At the preset time point of 1h, 6h, 12h, 24h, 48h and 72h, respectively, 3 rats were taken from each group, and 6 specimens were collected from the upper, middle and lower lungs respectively. The ultrastructure of the two groups were examined by HE staining. The optimal dose of paraquat 35mg.kg-1 was chosen as a stable and reliable model for the follow-up CT perfusion to chemically induced lung injury. To study the evaluation of early hemodynamic changes in patients with chemical lung injury by volume perfusion with 320 slice CT, and compared with pathology. To explore the value of CT perfusion CT perfusion in monitoring and diagnosis of early functional changes in chemical lung injury. Thirty New Zealand white rabbits were divided into two groups: control group and experimental group. The optimal dose of paraquatine (PQQ) was 35mg 路kg / kg, and 320-slice CT perfusion scan was performed at 2 h ~ 6 h ~ 12 h ~ 24 h ~ 48 h. At the above time point, 4 lung tissues were killed by air embolization for pathological examination.) the mean values of rBF- rBV and rPS in the control group were 128.54 卤31.31 卤25.20 卤4.15 ~ 13.06 卤3.05 ~ 2 respectively) there was a significant correlation between the changes of lung CTP parameters and pathological changes in the experimental group. There was a negative correlation between capillary endothelial cell swelling and blood flow. There was a positive correlation between the degree of capillary basement membrane damage and vascular permeability. After administration of paraquat, the pulmonary blood flow decreased gradually with the passage of time, and the analysis of variance showed that 24 hours blood flow was significantly lower than that at 0 hour after administration of paraquat (P 0.001). After paraquat administration, the pulmonary vascular permeability increased gradually with time, and the variance analysis showed that the blood vessel permeability at 2 hours was significantly different from that at 0 hour after administration of paraquat (P 0.001). The relationship between the plasma TGF- 尾 1 content and the perfusion parameter: PS. The relationship between the content of TNF-a and the perfusion parameter was 0.68520.05.The results showed that the relationship between the plasma TNF-a content and the perfusion parameter PS was 0.385620.050.385620.05. the relationship between the content of plasma TGF- 尾 _ 1 and the perfusion parameter was 0.385620.05.The results showed that the relationship between plasma TGF- 尾 _ 1 content and perfusion parameter PS was 0.385620.05. There was no statistical difference between the two groups, which indicated that there was no significant correlation between plasma TGF- 尾 1 TNF-a and perfusion parameter PS. CTP parameters (especially PS1) could reflect the abnormal hemodynamic changes in the early stage of chemical lung injury induced by paraquat. It is expected to be used for early prediction and dynamic monitoring of early lung injury.
【学位授予单位】：河北北方学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563

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