人羊膜上皮细胞气管内滴注对脂多糖诱导小鼠急性肺损伤的保护作用

发布时间：2018-05-27 04:47

本文选题：急性肺损伤 + 脂多糖　；参考：《细胞与分子免疫学杂志》2017年01期

【摘要】：目的探讨人羊膜上皮细胞(hAEC)对脂多糖(LPS)诱导的急性肺损伤小鼠的治疗作用及其相关机制。方法雄性C57BL/6小鼠随机分成正常对照组、hAEC对照组、LPS诱导的急性肺损伤组和hAEC治疗组,每组10只。后2组小鼠经口咽插管注入LPS(5 mg/kg),1 h后,再分别注入PBS(50μL)或1×107个/m L hAEC(50μL);正常对照组和hAEC对照组则经口咽插管注入等量PBS或hAEC。LPS处理24 h后处死小鼠,收集血液及肺脏组织,提取肺组织细胞质/细胞核蛋白,另取肺组织进行石蜡包埋、切片;称量并计算肺组织湿/干质量(W/D)比;ELISA检测血清中白细胞介素10(IL-10)、IL-6、IL-1β及肿瘤坏死因子α(TNF-α)含量;HE染色观察肺组织病变,Western blot法检测肺组织核转录因子κBp65(NF-κBp65)蛋白的表达。结果与正常对照组相比,LPS处理后小鼠肺组织W/D比增大,血清中IL-6、IL-1β及TNF-α含量增加,而IL-10含量减少;肺组织微血管破坏,发生肺水肿,肺泡间隔增厚;肺组织中NF-κBp65蛋白活性增加。hAEC处理使肺组织病变减轻,W/D比减小,血清中IL-6、IL-1β及TNF-α含量减少,肺组织NF-κBp65蛋白活性降低。结论 hAEC可降低肺组织中NF-κBp65的活性,显著减轻LPS诱导的急性肺损伤。
[Abstract]:Objective to investigate the therapeutic effect of human amniotic epithelial cells (hAECs) on acute lung injury induced by lipopolysaccharide (LPS) in mice and its related mechanisms. Methods male C57BL/6 mice were randomly divided into control group (n = 10) and hAEC group (n = 10). The mice in the latter two groups were injected with LPS(5 mg 路kg ~ (-1) 渭 L or 1 脳 10 ~ 7 PBS(50 渭 L hAEC(50 渭 L after oral pharyngeal intubation for 1 h, while those in the normal control group and hAEC control group were treated with the same amount of PBS or hAEC.LPS for 24 h, and the blood and lung tissues were collected. Lung cytoplasm / nuclear protein was extracted and paraffin embedded and sectioned. The lung tissue wet / dry weight (W / D) ratio was used to detect the levels of IL-6 尾 and TNF- 伪 in serum by Elisa. The expression of NF- 魏 B p65 NF- 魏 B p65 protein in lung tissue was detected by Western blot method. Results compared with the control group, the W / D ratio of lung tissue increased and the contents of IL-6 / IL-1 尾 and TNF- 伪 increased, while the content of IL-10 decreased, the pulmonary microvessel was destroyed, pulmonary edema and alveolar septum thickening occurred in the lung tissue treated with lipopolysaccharide, and the contents of IL-1 尾 and TNF- 伪 in serum were increased. The increase of NF- 魏 Bp65 protein activity in lung tissue reduced the WR / D ratio of lung tissue, decreased the contents of IL-1 尾 and TNF- 伪 in serum, and decreased the activity of NF- 魏 Bp65 protein in lung tissue. Conclusion hAEC can decrease the activity of NF- 魏 Bp65 in lung tissue and attenuate the acute lung injury induced by LPS.
【作者单位】：第四军医大学附属唐都医院胸腔外科;陕西省人民医院血管外科;
【基金】：陕西省自然科学基础研究计划项目(2014JM2-8144)
【分类号】：R563

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