菠萝蛋白酶诱导小鼠急性过敏性气道炎症的作用研究

发布时间：2018-05-30 16:48

本文选题：菠萝蛋白酶 + 型固有淋巴细胞　；参考：《现代免疫学》2017年04期

【摘要】：探讨菠萝蛋白酶诱导小鼠急性过敏性气道炎症中2型固有淋巴细胞(group 2innate lymphoid cell,ILC2)及相关细胞因子的变化及意义。对未致敏BALB/c雌性小鼠在第1天和第3天给予菠萝蛋白酶滴鼻,ELISA检测12h内小鼠肺组织匀浆上清中IL-33变化,5d后处死小鼠,肺组织切片病理观察,瑞氏染色计数BALF中嗜酸性粒细胞含量,流式检测肺组织中ILC2数量变化,ELISA检测BALF中IL-5、IL-13水平。结果显示,菠萝蛋白酶诱导气道上皮细胞产生IL-33,3h时开始,6h时达高峰;菠萝蛋白酶滴鼻小鼠肺组织切片显示大量炎性细胞浸润和杯状细胞黏液分泌增加,BALF中嗜酸性粒细胞比例增加,IL-5和IL-13含量增加,流式检测肺组织中ILC2数量增多。研究表明,菠萝蛋白酶刺激小鼠气道上皮细胞释放IL-33,引起小鼠肺ILC2活化增殖并分泌大量Th2型细胞因子,引起嗜酸性粒细胞浸润等急性过敏性气道炎症表现,提示ILC2促进早期急性过敏性气道炎症的发生。
[Abstract]:To investigate the changes and significance of type 2 inherent lymphocyte group 2innate lymphoid cell2 (ILC2) and related cytokines in acute allergic airway inflammation induced by bromelain in mice. The unsensitized BALB/c female mice were killed on the 1st and 3rd day by using bromelain nasal drip Elisa to detect the changes of IL-33 in the lung tissue supernatant of the mice within 12 hours. The eosinophilic granulocytes in BALF were counted by Rayleigh staining and the changes of ILC2 in lung tissue were detected by flow cytometry. IL-5 and IL-13 in BALF were detected by Elisa. The results showed that the production of IL-33 was induced by bromelain and reached the peak at the beginning of 6h. The lung tissue sections of mice fed with bromelain showed that a large number of inflammatory cells infiltrated and goblet cell mucus secretion increased. The percentage of eosinophils increased in BALF and the contents of IL-5 and IL-13 were increased. The number of ILC2 in lung tissue was increased by flow cytometry. The results showed that bromelain stimulated the release of IL-33 from mouse airway epithelial cells, induced the activation and proliferation of ILC2 and the secretion of a large number of Th2 cytokines in the lung of mice, and caused the infiltration of eosinophilic granulocytes and other acute allergic airway inflammation. The results suggest that ILC2 promotes the occurrence of early acute allergic airway inflammation.
【作者单位】：郑州大学护理学院基础教研室;河南科技大学医学院;郑州大学基础医学院微生物与免疫学系;
【分类号】：R562.25

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