单肺通气预处理对单肺通气大鼠非通气侧肺损伤的影响

发布时间：2018-06-02 14:09

本文选题：单肺通气 + 肺损伤　；参考：《山东医药》2017年38期

【摘要】：目的单肺通气预处理对单肺通气大鼠非通气侧肺损伤的影响,并探讨其机制。方法 40只SD大鼠,随机分为C组、O组、DM组、Y组各10只。C组双肺通气2.5 h;O组实施右侧单肺通气2 h后恢复双肺通气0.5 h,DM组实施双甲基草基甘氨酸(DMOG)预处理,通气方法同O组;Y组实施右侧单肺通气预处理,通气方法同O组。分别于单肺通气结束(T1)及实验结束(T2)游离并切取左肺下叶组织测定肺湿干重比(W/D)并在光镜下评价肺损伤情况,T2时采用羟胺法测定超氧化物歧化酶(SOD)、硫代巴比妥酸法测定丙二醛(MDA)和髓过氧化物酶(MPO)含量,T1、T2时采用Western blotting法检测低氧诱导因子1α(HIF-1α)和血红素加氧酶-1(HO-1)蛋白表达。结果与同组T1时相比,O组、DM组、Y组T2时W/D及肺损伤评分均升高,HIF-1α和HO-1表达均降低(P均0.05)。与C组同时点相比,O组、DM组、Y组的W/D、肺损伤评分及HIF-1α、HO-1蛋白表达均升高(P均0.05)。与O组同时点相比,DM组、Y组W/D及肺损伤评分均降低,HIF-1α和HO-1表达均升高(P均0.05)。与C组相比,O组、DM组、Y组SOD含量降低,MDA和MPO含量升高(P均0.05)。与O组相比,DM组、Y组SOD含量升高,MDA和MPO含量降低(P均0.05)。结论单肺通气预处理可减轻单肺通气大鼠非通气侧肺损伤,其机制可能与上调肺组织SOD、HIF-1α、HO-1表达并下调MDA和MPO表达有关。
[Abstract]:Objective to investigate the effects of single lung ventilation preconditioning on non-ventilatory lung injury in rats. Methods Forty Sprague-Dawley rats were randomly divided into two groups: group C (n = 10), group C (n = 10) and group C (n = 10). Group A (n = 10) received two-lung ventilation for 2.5 h and group O (n = 5) received two-lung ventilation for 2 h, and group DM (n = 5) received DMOG pretreatment after 0.5 h of dual lung ventilation. The ventilation method was the same as that in group O, and the right single lung ventilation was performed in group Y, and the ventilation method was the same as that in group O. T _ 1) and T _ 2) were dissociated and removed from the left lower lobe of lung to determine the lung wet / dry weight ratio (W / D) and to evaluate lung injury under light microscope. The superoxide dismutase (SOD) and thiobarbituric acid were determined by hydroxylamine method. The expression of hypoxia inducible factor-1 伪 (HIF-1 伪) and heme oxygenase-1 (HO-1) protein were detected by Western blotting method in order to determine the content of malondialdehyde (MDA) and myeloperoxidase (MPO). Results compared with the same group at T1, the expression of HIF-1 伪 and HO-1 decreased significantly in T 2 group and lung injury score (P < 0.05). Compared with group C at the same time, the WR, lung injury score and HIF-1 伪 -HO-1 protein expression were all increased (P 0.05). Compared with O group at the same time, the expression of HIF-1 伪 and HO-1 increased significantly in both groups (P < 0.05). Compared with group C, the content of SOD decreased and the content of MPO increased (P < 0.05). Compared with O group, the content of SOD increased and the content of MPO decreased (P < 0.05). Conclusion One-lung ventilation preconditioning can alleviate the injury of non-ventilatory lung in rats with single lung ventilation, and its mechanism may be related to the up-regulation of the expression of MDA HIF-1 伪 and down-regulation of the expression of MDA and MPO in lung tissue.
【作者单位】：河南省人民医院;
【基金】：河南省郑州市重点科技攻关项目(131PPTGG379-4)
【分类号】：R563

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