IL-17基因缺失对小鼠重症哮喘肺部炎性反应的影响

发布时间：2018-06-17 18:59

本文选题：重症哮喘 + IL-　；参考：《中山大学学报(医学科学版)》2014年06期

【摘要】：【目的】探讨IL-17基因缺失对小鼠重症哮喘肺部炎性反应的影响。【方法】通过鼻腔给予纯化尘螨提取物对野生型(WT)和IL-17基因敲除(IL-17KO)小鼠建立重症哮喘模型,造模结束后,收集支气管肺泡盥洗液(BALF)及肺组织。通过免疫组化染色观察IL-17在WT小鼠肺组织中的表达情况。检测BALF中细胞数量、分型(包括巨噬细胞、中性粒细胞、淋巴细胞、嗜酸性粒细胞和上皮细胞)。通过ELISA测定BALF中IL-17、IL-4、IL-5、IL-13、TNF-α、IFN-γ、KC等细胞因子的水平。【结果】通过尘螨提取物的长期慢性刺激可成功制备重症哮喘模型,而且在模型小鼠中的IL-17表达明显增高。与WT模型小鼠相比较,IL-17KO模型小鼠中的巨噬细胞、中性粒细胞、淋巴细胞数量明显减少,Th2细胞因子包括IL-4和IL-5以及促炎性细胞因子KC水平明显降低。【结论】IL-17可通过募集炎性细胞以及调控Th2型细胞因子和KC的产生而在重症哮喘的肺部炎性反应中起重要作用。
[Abstract]:[objective] to investigate the effect of IL-17 gene deletion on pulmonary inflammatory response in mice with severe asthma. [methods] the model of severe asthma was established in mice with wild type WTand IL-17 gene knockout by nasal administration of purified dust mite extract. Bronchoalveolar lavatory fluid (BALF) and lung tissue were collected. The expression of IL-17 in lung tissue of WT mice was observed by immunohistochemical staining. Cell count and typing (including macrophages, neutrophils, lymphocytes, eosinophilic granulocytes and epithelial cells) in BALF were detected. The levels of cytokines such as IL-17, IL-4, IL-5, IL-13, TNF- 伪 and IFN- 纬 -KC in BALF were determined by Elisa. [results] the model of severe asthma could be successfully established by chronic stimulation of dust mite extract, and the expression of IL-17 in model mice was significantly increased. Macrophages and neutrophils in IL-17KO model mice were compared with WT model mice. Th2 cytokines, including IL-4, IL-5 and pro-inflammatory cytokine KC, were significantly reduced in lymphocytes. [conclusion] IL-17 may play an important role in severe disease by recruiting inflammatory cells and regulating the production of Th2 cytokines and KC. Lung inflammation plays an important role in asthma.
【作者单位】：西安交通大学医学院第一附属医院呼吸内科;西安交通大学生命科学与技术学院与前沿科学与技术研究院转化医学研究中心;
【基金】：中央高校基本科研业务费专项资金(08143010)
【分类号】：R562.25

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