大鼠肺缺血再灌注损伤对瞬时受体电位香草酸亚型1在肺和脑干表达的影响

发布时间：2018-06-20 01:30

本文选题：缺血再灌注 + 瞬时受体电位香草酸亚型　；参考：《生物医学工程学杂志》2017年01期

【摘要】：本研究目的是探讨大鼠肺缺血再灌注损伤对瞬时受体电位香草酸亚型1(TRPV1)在肺和脑干表达的影响。将16只SD大鼠随机分为假手术组(S组)和缺血再灌注组(IR组)。于缺血前、再灌注0.5 h及再灌注4 h抽取动脉血进行血气分析,观察动脉血氧分压(Pa O_2)及肺泡动脉氧分压差(A-a DO_2)的变化。实验结束时取左肺下叶测量氧化应激指标丙二醛(MDA)、炎症反应指标髓过氧化物酶活性(MPO)、神经肽降钙素基因相关肽(CGRP)和P物质(SP)。取左肺上叶和脑干检测TRPV1的基因和蛋白的表达。与S组相比,再灌注0.5 h和4 h时,IR组Pa O_2明显降低(P0.05),A-a DO_2明显升高(P0.05);IR组肺组织匀浆MPO活力及MDA含量均明显增高(P0.05);缺血再灌注使得CGRP含量明显增加(P0.05),而两组间SP含量未见明显差异。IR组TRPV1m RNA及蛋白在肺组织中表达明显上调(P0.05),而在脑干中的表达与S组比较无明显差异。本研究提示肺缺血再灌注损伤使TRPV1在肺组织中表达增加,CGRP释放增加。
[Abstract]:The aim of this study was to investigate the effects of pulmonary ischemia-reperfusion injury on the expression of vanillic acid subtype 1 TRPV1 in the lung and brain stem of rats. Sixteen SD rats were randomly divided into sham operation group (S group) and ischemia reperfusion group (IR group). Before ischemia, 0.5 h after reperfusion and 4 h after reperfusion, arterial blood was collected for gas analysis, and the changes of arterial partial pressure of oxygen (PaO2) and alveolar arterial oxygen pressure difference (A- a DO2) were observed. At the end of the experiment, the indexes of oxidative stress (MDA), myeloperoxidase (MPO), neuropeptide calcitonin gene-related peptide (CGRPP) and substance P (substance P) were measured at the end of the experiment. The gene and protein expression of TRPV1 was detected in the upper lobe of left lung and brain stem. Compared with group S, At 0.5 h and 4 h after reperfusion, PaO2 in IR group significantly decreased the activity of MPO and the content of MDA in lung homogenate of P0.05 group increased significantly, while the content of CGRP increased significantly in group P0.05 and reperfusion, but there was no significant difference in SP content between the two groups. The expression of TRPV1 mRNA and protein in the lung tissue was up-regulated in IR group, but there was no significant difference in the expression of TRPV1 mRNA and protein in brain stem compared with that in S group. This study suggested that the expression of TRPV1 increased in lung tissue and CGRP release increased after lung ischemia reperfusion injury.
【作者单位】：重庆医科大学附属第一医院麻醉科;四川大学华西医院麻醉科;
【基金】：国家自然科学基金项目(81170077) 卫生部国家临床重点专科建设项目(财社〔2011〕170号) 重庆市医学重点学科建设项目(渝卫科教〔2007〕2号)
【分类号】：R56

【相似文献】