帕夫林通过抑制NLRP3形成减轻博莱霉素诱导的小鼠肺纤维化

发布时间：2018-06-21 16:03

本文选题：帕夫林 + 肺纤维化　；参考：《南京医科大学学报(自然科学版)》2017年07期

【摘要】：目的:探讨帕夫林(paeoniflorin,PA)对博莱霉素(bleomycin,BLM)诱导的小鼠肺纤维化的影响及其作用机制。方法:将60只SPF级雄性C57BL/6小鼠随机分为4组:对照组(sham operation,SH)、对照组+帕夫林组(SH+PA)、模型组(BLM)、模型组+帕夫林(BLM+PA);气管内滴注博莱霉素(2 mg/kg)建立小鼠肺纤维化模型,对照组气管内滴注生理盐水,从造模当天开始,SH+PA、BLM+PA组小鼠每日给予50 mg/kg帕夫林灌胃,SH、BLM组小鼠每日给予等量生理盐水灌胃;在造模第14天处死小鼠,留取小鼠肺组织。记录各组小鼠体重变化;统计各组小鼠生存率;HE染色法观察小鼠肺组织纤维化程度;肺组织匀浆羟脯氨酸定量评估肺组织胶原蛋白含量;ELISA法测定肺组织匀浆中白介素(interleukin,IL)-18、IL-1β含量;Western blot法检测肺组织匀浆中NLRP3、转化生长因子β1(transforming growth factor-β1,TGF-β1)表达水平。结果:与对照组相比,帕夫林可显著提高小鼠生存率;经帕夫林治疗14 d后,小鼠肺组织纤维化病变较模型组减轻;小鼠肺组织胶原蛋白含量显著降低(P0.05);小鼠外周血及肺组织中IL-18、IL-1β含量较模型组相比明显减少(P0.05);与模型组相比,NLRP3蛋白水平以及TGF-β1蛋白表达水平显著下调(P0.05)。结论:帕夫林可减轻博莱霉素诱导的小鼠肺纤维化程度,其作用可能是通过抑制NLRP3炎症小体的形成发挥的。
[Abstract]:Aim: to investigate the effect of paeoniflorin (PAA) on pulmonary fibrosis induced by bleomycin (BLM) in mice and its mechanism. Methods: sixty SPF male C57BL / 6 mice were randomly divided into four groups: control group (sham operation), control group (Paflin group), SH-PAA group, model group, bleomycin group (2 mg 路kg ~ (-1) 路kg ~ (-1), intratracheal instillation of bleomycin (2 mg 路kg ~ (-1). Mice in the control group were given 50 mg/kg Pavlin intragastric perfusion of normal saline daily, and the mice in the control group were killed on the 14th day, and the lung tissue of the mice was retained. The mice in the control group were given normal saline intratracheal drip, and the mice in the SH-PA-BLM group were given 50 mg/kg Pavlin intragastric perfusion daily, and the mice in the control group were killed on the 14th day of the model. The changes of body weight in each group were recorded, the survival rate of each group was counted and the degree of pulmonary fibrosis was observed by HE staining. Lung tissue homogenate hydroxyproline quantitative evaluation of collagen content in lung tissue. Elisa method was used to determine the content of interleukin-IL-18 and IL-1 尾 in lung homogenate. Western blot method was used to detect the expression of NLRP3 and TGF- 尾 1 in lung homogenate of lung tissue homogenate, and the expression of transforming growth factor 尾 1(transforming growth factor- 尾 1 (TGF- 尾 1) in lung homogenate was determined by Elisa. Results: compared with the control group, the survival rate of mice was significantly improved by Paflin, and the pathological changes of pulmonary fibrosis in the model group were alleviated after 14 days of treatment with Paflin. Compared with the model group, the contents of IL-18 and IL-1 尾 in the peripheral blood and lung tissues of the mice decreased significantly, while the level of NLRP3 protein and the expression of TGF- 尾 1 in the model group decreased significantly compared with the model group, and the expression level of TGF- 尾 1 protein decreased significantly compared with the model group. Conclusion: Pavlin can attenuate the degree of pulmonary fibrosis induced by bleomycin in mice, which may play a role in inhibiting the formation of NLRP3 inflammatory corpuscles.
【作者单位】：东南大学附属中大医院呼吸内科;南京医科大学第一附属医院呼吸与危重症医学科;
【基金】：国家自然科学基金(81273571) 江苏省呼吸病临床医学研究中心(BL2012012)
【分类号】：R563

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