小青龙汤对哮喘小鼠气道变应性炎症作用的研究

发布时间：2018-06-21 23:45

本文选题：哮喘 + 小青龙汤　；参考：《重庆医学》2014年17期

【摘要】：目的探讨小青龙汤对卵蛋白致敏/激发小鼠气道变应性炎症的作用。方法 BALB/c小鼠以OVA致敏/激发,在其每日供水时加入小青龙汤;在最后一次激发24h后留取标本,检测肺泡灌洗液(BALF)中细胞计数和分类,测定支气管BALF、血清中细胞因子以及外周血和BALF中Foxp3+CD4+T细胞的比例。结果哮喘组可见明显炎性细胞浸润,经小青龙汤干预后肺部炎性细胞浸润显著减少;哮喘组BALF中炎性细胞,特别是嗜酸性粒细胞计数较对照组明显升高(P0.01);白细胞介素(IL)-4、IL-5水平较对照组明显升高(P0.01),Foxp3+T细胞比例显著下降(P0.01),Th1细胞因子干扰素(IFN)-γ无显著变化,治疗组与哮喘组比较,IL-4、IL-5水平、Foxp3+T细胞比例增加,差异无统计学意义(P0.05);IL-10水平显著升高。结论小青龙汤可有效抑制哮喘模型小鼠气道变应性炎症,其作用途径可能是通过诱导Foxp3+调节性T细胞产生、IL-10的分泌,进而抑制了Th2型免疫反应。
[Abstract]:Objective to investigate the effect of Xiao Azure Dragon decoction on allergic airway inflammation induced by ovalbumin in mice. Methods BALB / c mice were sensitized / stimulated by OVA and added small Azure Dragon decoction in their daily water supply, and the cells count and classification in alveolar lavage fluid (BALF) were determined 24 hours after the last stimulation. The ratio of cytokines in bronchial BALF, serum and Foxp3 CD4 T cells in peripheral blood and BALF were measured. Results the infiltration of inflammatory cells in asthma group was obvious, and the infiltration of inflammatory cells in lung was decreased after intervention of Xiao Azure Dragon decoction. In particular, the eosinophil count was significantly higher than that in the control group (P0.01), and the level of interleukin-4 (IL-5) was significantly higher than that in the control group (P0.01). The percentage of Foxp3 T cells decreased significantly (P0.01) and the number of Th1 cytokines interferon 纬 (IFN- 纬) did not change significantly. Compared with asthma group, the ratio of IL-4 / IL-5 and Foxp3 T cells increased in the treatment group, but there was no significant difference (P0.05) in the level of IL-10. Conclusion Xiao Azure Dragon decoction can effectively inhibit allergic airway inflammation in asthmatic mice, which may be through inducing the secretion of IL-10 by Foxp3 regulatory T cells, and thus inhibit the Th2 type immune response.
【作者单位】：成都军区总医院呼吸内科;第三军医大学新桥医院全军呼吸内科研究所;第三军医大学大坪医院野战外科研究所呼吸内科;
【基金】：国家自然科学基金资助项目(30900657) 成都军区总医院院管基金资助项目(424121B7)
【分类号】：R562.25

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