铜绿假单胞菌慢性肺部感染合并免疫低下大鼠模型的建立

发布时间：2018-06-22 15:09

本文选题：铜绿假单胞菌 + 慢性肺部感染　；参考：《中国医院药学杂志》2017年24期

【摘要】：目的:建立铜绿假单胞菌(Pseudomonas aeruginosa,PA)慢性肺部感染合并免疫低下大鼠模型,为相关模型建立与研究提供依据。方法:将实验大鼠分为空白对照组(A组)、肺部感染组(B组)、免疫低下组(C组)、肺部感染合并免疫低下组(D组)。以40 mg·kg~(-1)的剂量给予C、D2组大鼠腹腔注射环磷酰胺(cyclophosphamide,CTX),连续给药3 d,造模成功后以相同剂量每周注射一次;运用气管插管法,构建B、D组大鼠慢性肺部感染。在模型建立期间,以固定时间对大鼠肺载菌量、肺部病理学变化、免疫学指标变化、T细胞亚群等指标进行考察。结果:HE染色结果显示,B、D组大鼠在造模成功后肺部产生病理学病变,并有较明显的中性粒细胞聚集等现象。与A组相比,B组与D组大鼠外周血中性粒细胞有不同程度的增高,C组与D组白细胞数目有所下降,实验组大鼠CD_4~+/CD_8~+有显著增高。结论:通过该实验方法,成功建立PA慢性肺部感染合并免疫低下大鼠模型,成功率高、死亡率低,适于采取该方法进行相关研究。
[Abstract]:Objective: to establish a rat model of chronic pulmonary infection of Pseudomonas aeruginosa PA with hypoimmunity, and to provide evidence for the establishment and study of the model. Methods: the experimental rats were divided into blank control group (group A), pulmonary infection group (group B), hypoimmunity group (group C) and pulmonary infection combined with hypoimmunity group (group D). The rats in the 40 mg kg ~ (-1) group were given cyclophosphamide CTX intraperitoneally for 3 days, and then the rats were injected with the same dose once a week after the successful establishment of the model, and the chronic pulmonary infection was constructed by tracheal intubation. During the establishment of the model, fixed time was used to investigate the changes of lung bacterial load, lung pathology, immunological indexes and T cell subsets in rats. Results the pathological changes of lung and neutrophil aggregation were found in the lung of rats in group B and D after the successful establishment of the model. Compared with group A, neutrophils in peripheral blood of rats in group B and group D increased to some extent, and the number of leukocytes in group C and group D decreased, and CD4 ~ / CD8 ~ increased significantly in experimental group. Conclusion: the model of PA chronic pulmonary infection combined with hypoimmunity was successfully established by this method, with high success rate and low mortality rate.
【作者单位】：中国医科大学附属盛京医院药学部;
【基金】：辽宁省自然科学基金项目(编号:2014021090)
【分类号】：R-332;R563.1

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