lncRNA在结核病患者外周血的差异性表达

发布时间：2018-06-25 12:24

  本文选题：肺结核 + 结核分枝杆菌潜伏感染　； 参考：《中国病原生物学杂志》2017年04期

【摘要】：目的比较活动性肺结核患者、潜伏感染者与健康对照者外周血结核分枝杆菌特异性抗原刺激前后lncRNA1和lncRNA2的差异性表达,探讨其作为诊断结核分枝杆菌感染生物标志物的可能性。方法收集活动性肺结核患者、潜伏感染者和健康对照者全血,分别在特异性抗原刺激前后提取RNA,利用实时荧光定量PCR方法检测lncRNA1和lncRNA2的mRNA表达水平。应用ELISA检测血浆中IFN-γ和IL-2含量。结果结核分枝杆菌特异性抗原刺激前,结核病组、潜伏感染组全血lncRNA1相对表达量较健康对照组分别下降32%和64%;lncRNA2结核病组相对表达量是健康对照组的6.10倍;抗原刺激后,结核病组、潜伏感染组lncRNA1相对表达量较健康对照组分别下降33%和77%,lncRNA2潜伏感染组相对表达量较健康对照组下降84%。与刺激前相比,抗原刺激后健康对照组、结核病组lncRNA1相对表达量分别提高1.80倍和2.80倍,lncRNA2健康对照组、潜伏感染组分别提高3.50倍和1.86倍(P0.05)。健康组与潜伏感染组、结核病组与潜伏感染组、健康组与结核病组lncRNA1ROC曲线下面积(AUC)分别为0.700、0.752和0.400,lncRNA2的AUC分别为0.702、0.465和0.762。在结核特异性蛋白刺激前,结核病组IFN-γ、IL-2含量均高于健康组(P0.05),刺激后,结核病组及潜伏感染组与健康组相比较、潜伏感染组与结核病组相比较IFN-γ均增高(P0.05)。与刺激前相比,刺激后3组IFN-γ、IL-2诱导表达量均显著增高(P0.05)。结论活动性结核病、潜伏感染和健康对照者全血结核特异性抗原刺激前后lncRNA1和lncRNA2均出现差异性表达,结核病及潜伏感染者血浆IFN-γ含量增高。lncRNA1和lncRNA2可能是诊断肺结核感染的潜在生物标志物。
[Abstract]:Objective to compare the differential expression of lncRNA1 and lncRNA2 between the latent infection and the healthy controls before and after the specific antigen stimulation of Mycobacterium tuberculosis in the peripheral blood, and to explore the possibility of it as a biomarker for the diagnosis of Mycobacterium tuberculosis. Methods the methods of collecting active tuberculosis, latent infection and health were collected. In the whole blood, RNA was extracted before and after the specific antigen stimulation, and the mRNA expression level of lncRNA1 and lncRNA2 was detected by real-time fluorescence quantitative PCR. The content of IFN- gamma and IL-2 in plasma was detected by ELISA. The results showed that the relative expression of lncRNA1 in the whole blood of the tuberculosis group and the latent infection group was more healthy before the specific antigen stimulation of Mycobacterium tuberculosis. The relative expression of lncRNA2 in the lncRNA2 group was 6.10 times as high as that in the healthy control group. After the antigen stimulation, the relative expression of lncRNA1 in the tuberculosis group and the latent infection group was 33% and 77%, respectively, and the relative expression of the lncRNA2 latent infection group was lower than that of the healthy control group, 84%. was compared with the pre stimulation, and the antigen stimulation was healthy. In the control group, the relative expression of lncRNA1 in the tuberculosis group was increased by 1.80 times and 2.80 times respectively. The lncRNA2 healthy control group, the latent infection group increased 3.50 times and 1.86 times (P0.05) respectively. The health group and the latent infection group, the tuberculosis group and the latent infection group, the area under the lncRNA1ROC curve of the health group and the tuberculosis group (AUC) were 0.700,0.752 and 0.400, lncRN, respectively. The AUC of A2 was 0.702,0.465 and 0.762. before the tuberculosis specific protein stimulation, and the IFN- gamma and IL-2 content in the tuberculosis group were all higher than that of the healthy group (P0.05). After the stimulation, the tuberculosis group and the latent infection group compared with the healthy group, the latent infection group compared with the tuberculosis group, the IFN- gamma increased (P0.05). Compared with the stimulation, the 3 groups of IFN- gamma, IL-2 induction were compared with the stimulation before the stimulation. The expression of lncRNA1 and lncRNA2 in active tuberculosis, latent infection and healthy controls were all differentially expressed before and after tuberculosis specific antigen stimulation. The increase of IFN- gamma content in the plasma of tuberculosis and latent infection.LncRNA1 and lncRNA2 may be a potential biomarker for the diagnosis of tuberculosis infection.
【作者单位】： 石河子大学医学院病原微生物学与免疫学教研室;北京大学医学部基础医学院医学信息学系;
【基金】：国家"十二五"计划重大传染病防治专项结核病专项(No.2013ZX10003003-002-002)
【分类号】：R521
，

本文编号：2065905