白介素33在百草枯诱导小鼠急性肺损伤中的作用机制研究

发布时间：2018-06-27 13:10

本文选题：白介素 + 百草枯　；参考：《安徽医科大学学报》2014年08期

【摘要】：目的探讨白介素33(IL-33)在百草枯(PQ)染毒小鼠急性肺损伤(ALI)模型中的表达及作用。方法 25只SPF级雌性BALB/c小鼠平均分为正常对照组,PQ损伤6、12、24、48 h组。腹腔注射PQ(30 mg/kg)建立小鼠ALI模型并于各时间点处死取材。评价肺组织病理变化和炎症细胞浸润;观察血清白介素1β(IL-1β)、IL-2、IL-6、肿瘤坏死因子α(TNF-α)和肺组织髓过氧化物酶(MPO)水平变化;RTPCR、免疫组化和Western blot分别检测组织中IL-33 mRNA和蛋白表达水平变化。分析IL-33与病理损伤和炎症指标关联性以明确IL-33在调解内源性细胞因子及在ALI中的作用。结果与正常对照组相比,PQ损伤后小鼠肺组织弥漫性损伤伴有严重水肿及大量炎症细胞浸润,促炎细胞因子IL-1β、IL-6、TNF-α、MPO明显升高,抑炎细胞因子IL-2明显下调(P0.05);PQ损伤后IL-33在mRNA和蛋白表达水平均显著下调(P0.05)。关联性分析显示IL-33与病理损伤指标,IL-1β、IL-6、TNF-α、MPO均呈明显负相关(P0.05),与IL-2呈正相关趋势,但差异无统计学意义。结论 IL-33在PQ诱导小鼠ALI中表达水平下降,提示可能在调解内源性炎症因子平衡中发挥重要作用,可为临床干预和治疗ALI的新靶点。
[Abstract]:Objective to investigate the expression and role of interleukin-33 (IL-33) in paraquat (PQ) induced acute lung injury (Ali) in mice. Methods 25 SPF female BALB / c mice were divided into normal control group (n = 60) with PQ injury (n = 6) and control group (n = 24) for 48 h. Ali model of mice was established by intraperitoneal injection of PQ (30 mg/kg) and killed at each time point. The changes of serum interleukin-1 尾 (IL-1 尾) IL-2IL-6, tumor necrosis factor- 伪 (TNF- 伪) and pulmonary myeloperoxidase (MPO) were evaluated. The expression of IL-33 mRNA and protein were detected by immunohistochemistry and Western blot, respectively. To analyze the relationship between IL-33 and pathological injury and inflammation in order to clarify the role of IL-33 in mediating endogenous cytokines and Ali. Results compared with the control group, the diffuse injury of lung tissue was accompanied by severe edema and a large number of inflammatory cells infiltration, and the inflammatory cytokine IL-1 尾 and IL-6 TNF- 伪 MPO were significantly increased after PQ injury in mice. Anti-inflammatory cytokine IL-2 down-regulated (P0.05) IL-33 expression level in mRNA and protein after PQ injury significantly (P0.05). The correlation analysis showed that IL-33 was negatively correlated with IL-6 TNF- 伪 MPO (P0.05), and had a positive correlation with IL-2, but there was no significant difference between IL-33 and IL-6TNF- 伪 MPO. Conclusion the decrease of IL-33 expression in PQ induced Ali suggests that IL-33 may play an important role in mediating the balance of endogenous inflammatory factors and may be a new target for clinical intervention and treatment of Ali.
【作者单位】：安徽医科大学北京微生物流行病研究所;北京微生物流行病研究所病原微生物生物安全国家重点实验室;
【基金】：国家自然科学基金(编号:81371805) 国家重点基础研究发展计划(973)(编号:2012CB518905)
【分类号】：R595.4;R563.8

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