松弛素受体1在矽肺大鼠肺组织中表达研究

发布时间：2018-07-08 09:30

本文选题：矽肺 + 肺纤维化　；参考：《中国职业医学》2016年01期

【摘要】：目的探讨松弛素受体1(RXFP1)在石英尘致矽肺中的潜在作用。方法无特定病原体级雄性Wistar大鼠64只随机分为对照组和染尘组。采用非暴露式气管内一次性灌注法,染尘组予气管内灌入质量浓度为500 g/L德国标准石英混悬液0.1 m L,对照组予气管内灌入0.9%氯化钠溶液0.1 m L。2组大鼠分别在染毒第1、7、14和28天各处死8只,以苏木精-伊红染色观察大鼠肺组织的病理学变化,分别用实时荧光定量聚合酶链反应法和免疫组织化学法分析大鼠肺组织中Rxfp1 mRNA和RXFP1蛋白的表达。结果染尘第28天,染尘组大鼠双肺肉眼可见灰白色结节,光镜下可观察到肺泡间隔断裂,局部可见明显的矽结节。染尘组大鼠肺组织Rxfp1 mRNA相对表达水平于染尘第1天升高至对照组的145%(P0.01),于第7和14天下降至与对照组接近水平(P0.05),于第28天下降至对照组的45%(P0.01)。染尘组大鼠肺组织中RXFP1蛋白相对表达水平从染毒第7天开始高于对照组(P0.01),第28天达到最高水平(P0.01)。结论 RXFP1可能在抑制矽肺发病过程中发挥重要作用。
[Abstract]:Objective to investigate the potential role of relaxin receptor 1 (RXFP1) in silica dust-induced silicosis. Methods Sixty-four male Wistar rats without specific pathogens were randomly divided into two groups: control group and dust-exposed group. Non-exposed intratracheal instillation was used. In the dust-exposed group, 0.1 mL German standard quartz suspension was injected into the trachea with a mass concentration of 500 g / L, and 8 rats in the control group were treated with 0.9% sodium chloride solution 0.1 m L.2 on the 1st day, 714 d and 28 d, respectively. The expression of Rxfp1 mRNA and RXFP1 protein in rat lung tissues were detected by real-time fluorescence quantitative polymerase chain reaction and immunohistochemistry. Results on the 28th day, gray and white nodules could be seen in both lungs of the rats in the dust-exposed group. The rupture of the alveolar septum was observed under light microscope. The relative expression of Rxfp1 mRNA in lung tissue of rats exposed to dust increased to 145% (P0.01) of the control group on the 1st day, decreased to the same level as the control group on the 7th and 14th days (P0.05), and decreased to 45% of the control group on the 28th day (P0.01). The relative expression of RXFP1 protein in the lung tissue of the dust-exposed group was higher than that of the control group from the 7th day (P0.01), and reached the highest level on the 28th day (P0.01). Conclusion RXFP1 may play an important role in inhibiting the pathogenesis of silicosis.
【作者单位】：深圳市龙岗区人民医院检验科;深圳市龙岗区疾病预防控制中心;华中科技大学公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室;
【基金】：国家自然科学基金青年基金(30800901) 深圳市科技计划项目(JCYJ20150402164314696) 深圳市龙岗区科技发展资金(YLWS20150513174008294);深圳市龙岗区科技发展基金(YLWS2015051114456591) 深圳市卫生计生系统科研项目(201402126)
【分类号】：R135.2

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