血清IL-5、IL-12在不同海拔COPD发病机制中的意义

发布时间：2018-07-11 15:13

本文选题：IL-5 + IL-12　；参考：《青海大学》2012年硕士论文

【摘要】：目的本文用ELISA法对不同海拔地区100例慢性阻塞性肺疾病（COPD）患者进行了血清IL-5、IL-12水平的测定，探讨它们在不同海拔COPD发病机制中的作用及意义。方法选择中海拔（2200m）AECOPD患者37例、缓解期患者31例和对照组30例，分别为中海拔AECOPD组、缓解期组和对照组，高海拔（4500m）COPD缓解期患者32例和对照组32例，分别为高海拔缓解期组和对照组，用ELISA法测定血清IL-5、IL-12水平。结果中海拔AECOPD组血清IL-5水平明显高于缓解期组和对照组，缓解期组血清IL-5水平低于对照组（P＜0.05）；高海拔缓解期组和对照组血清IL-5水平无差异（P＞0.05）；高海拔缓解期组和对照组血清IL-5水平均高于中海拔缓解期组和中海拔对照组（P＜0.05）。中海拔缓解期组血清IL-12水平高于AECOPD组和对照组（P＜0.05）；中海拔AECOPD组血清IL-12水平和对照组无差异（P＞0.05）；高海拔缓解期组血清IL-12水平高于对照组（P＜0.05）；高海拔缓解期组血清IL-12水平低于中海拔缓解期组（P＜0.05）；高海拔对照组和中海拔对照组血清IL-12水平无差异（P＞0.05）。中海拔AECOPD组FEV1与血清IL-5水平呈负相关（r=-0.744，，P 0.05），而FEV1与血清IL-12水平呈正相关（r=0.738，P 0.05）。结论1、AECOPD组血清IL-5水平高于缓解期组，血清IL-12水平低于缓解期组，提示血清IL-5参与COPD气道急性炎症反应，血清IL-12参与COPD气道损伤修复；2、高海拔COPD缓解期组和对照组血清IL-5水平高于中海拔缓解期组和对照组，高海拔COPD缓解期组血清IL-12水平低于中海拔COPD缓解期组，提示IL-5、IL-12参与了低氧高寒环境下COPD低氧适应过程；3、AECOPD组血清IL-5水平越高，患者FEV1越低，而血清IL-12则相反，提示IL-5和IL-12参与COPD气道改变。
[Abstract]:Objective to study the role of IL-5 and IL-12 in the pathogenesis of COPD in 100 patients with chronic obstructive pulmonary disease (COPD) at different elevations by Elisa. Methods Thirty-seven patients with moderate altitude (2200m) AECOPD, 31 patients in remission period and 30 patients in control group were selected as middle altitude AECOPD group, remission group and control group, 32 patients with COPD at high altitude (4500m) and 32 patients with COPD at high altitude (4500m). The serum levels of IL-5 and IL-12 were measured by Elisa in remission stage group and control group. Results the level of serum IL-5 in AECOPD group was significantly higher than that in remission group and control group (P < 0.05), but there was no difference between high altitude group and control group (P > 0.05). The levels of serum IL-5 in the remission period group and control group were higher than those in the middle altitude remission stage group and the middle altitude control group (P < 0.05). The level of serum IL-12 in the remission period group was higher than that in the AECOPD group and the control group (P < 0.05), the serum IL-12 level in the AECOPD group was not different from that in the control group (P > 0.05), the serum IL-12 level in the high altitude remission period group was higher than that in the control group (P < 0.05). The level of serum IL-12 in the remission period group was lower than that in the middle altitude group (P < 0.05), but there was no difference between the high altitude control group and the middle altitude control group (P > 0.05). There was a negative correlation between FEV1 and serum IL-5 level in AECOPD group (r = 0.744, P 0.05), but a positive correlation between FEV _ 1 and serum IL-12 level (r = 0.738, P 0.05). Conclusion 1the level of serum IL-5 in AECOPD group is higher than that in remission group, and the level of serum IL-12 is lower than that in remission group, suggesting that serum IL-5 is involved in acute airway inflammation of COPD, and serum IL-12 is involved in the repair of COPD airway injury. 2, the serum IL-5 level of COPD remission period and control group at high altitude is higher than that of middle altitude remission stage and control group, and the serum IL-12 level in high altitude COPD remission phase is lower than that in middle altitude COPD remission stage group, which suggests that IL-5 IL-12 is involved in the process of COPD hypoxia adaptation under hypoxia and alpine environment. 3 the higher the level of serum IL-5 in AECOPD group, the lower the level of FEV1, whereas the lower the level of serum IL-12, suggesting that IL-5 and IL-12 are involved in the airway changes of COPD.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R563.9

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