重症肺炎患者血液中类鼻疽伯克霍尔德菌的鉴定及其多位点序列分型

发布时间：2018-07-14 13:58

【摘要】：目的分离鉴定一位重症肺炎患者血液中的未知细菌并进行多位点序列分型(Multilocus sequence typing,MLST)。方法采用VITEK-2 compact微生物系统进行鉴定和药物敏感试验,PCR法扩增细茵的16S r DNA基因,通过测序并与Gen Bank中相关序列进行比对。采用MLST进行分型,BURST软件分析细菌的遗传变异。结果经VITEK-2 compact微生物系统初步鉴定为类鼻疽伯克霍尔德菌。16S r DNA PCR扩增,产物经测序与Gen Bank中序列比对,发现与类鼻疽伯克霍尔德菌CP012577.1序列有99%同源性,证实该菌为类鼻疽伯克霍尔德菌(Burkholderia pseudomallei)。MLST发现一个新的序列型(sequence type,ST)-ST1348,与来自巴布亚新几内亚分离株仅有一个基因的差异,且均可溯源至澳大利亚株。结论来源于急性感染患者血液中的未知细菌是类鼻疽伯克霍尔德菌,MLST分型发现一个新的ST序列-ST1348,该分离株与巴布亚新几内亚菌株ST246密切相关。
[Abstract]:Objective to isolate and identify the unknown bacteria in the blood of a severe pneumonia patient and Multilocus sequence typing (MLST). Methods the VITEK-2 compact microbiological system was used for identification and drug sensitivity test. The PCR method was used to amplify the R DNA gene of the 16S, and was sequenced and compared with the related sequence in Gen Bank. The genetic variation of bacteria was analyzed by MLST and BURST software. The results were preliminarily identified by VITEK-2 compact microbiological system as.16S R DNA PCR amplification of Burke and Holder bacteria, and the product was sequenced and compared with Gen Bank sequence. It was found to have 99% homology with the CP012577.1 sequence of Burke and Holder like bacteria, which confirmed that the bacteria was a kind of genus of the genus. Burkholderia pseudomallei (Burkholderia pseudomallei).MLST found a new sequence type (sequence type, ST) -ST1348, with only one gene from the isolated strains from Papua New Guinea, and traceable to the Australian strain. Conclusion the unknown bacteria from the blood of the acute infected patients were Burke Holder, the MLST score. A new ST sequence -ST1348 was found, which is closely related to Papua New Guinea strain ST246.
【作者单位】：江苏大学医学院;扬州市第三人民医院检验科;江苏大学附属武进医院检验科;江苏大学附属人民医院检验科;
【基金】：国家自然科学基金(81101227) 镇江市社会发展项目(SH2015040)
【分类号】：R563.1

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