烟草烟雾暴露对哮喘大鼠气道CCR6mRNA及其蛋白表达的影响
发布时间：2018-07-24 11:36
【摘要】：目的研究烟草烟雾暴露对支气管哮喘（简称哮喘）大鼠气道CCR6(Chemokinereceptor6)表达的影响，探讨吸烟诱发和加重哮喘气道炎症的免疫学机制。 方法雄性Wistar大鼠40只，随机分为对照组、烟雾暴露组、哮喘组和哮喘+烟雾暴露组，每组各10只。建立哮喘大鼠模型和哮喘大鼠烟草烟雾暴露模型，采集大鼠支气管肺泡灌洗液(BALF)行白细胞计数及细胞分类，苏木精-伊红（HE）染色观察肺组织病理学改变，采用逆转录-聚合酶链式反应(RT-PCR)法及免疫组织化学染色法检测各组大鼠气道CCR6mRNA及其蛋白的表达。 结果①哮喘组、哮喘+烟雾暴露组BALF中白细胞总数、嗜酸粒细胞、中性粒细胞均高于对照组和烟雾暴露组，，P均0.05；哮喘+烟雾暴露组BALF中白细胞总数和中性粒细胞高于哮喘组，嗜酸粒细胞低于哮喘组，P均0.05。②哮喘组、哮喘+烟雾暴露组CCR6mRNA及其蛋白表达水平均明显高于对照组和烟雾暴露组，P均0.01；哮喘+烟雾暴露组CCR6mRNA及其蛋白表达水平明显高于哮喘组，P均0.01。 结论气道粘膜CCR6mRNA及其蛋白高表达提示CCR6可能参与了哮喘大鼠气道炎症反应；烟雾暴露可通过促使气道CCR6mRNA及其蛋白表达水平升高，诱发和加重哮喘大鼠气道慢性炎症。
[Abstract]:Objective to study the effect of tobacco smoke exposure on the expression of CCR6 (Chemokinereceptor6) in airway of asthmatic rats, and to explore the immunological mechanism of airway inflammation induced and aggravated by smoking. Methods 40 male Wistar rats were randomly divided into control group, smoke exposure group, asthma group and asthma smoke exposure group with 10 rats in each group. The rat model of asthma and the model of tobacco smoke exposure in asthmatic rats were established. The white blood cell count and cell classification of bronchoalveolar lavage fluid (BALF) were collected and the pathological changes of lung tissue were observed by hematoxylin and eosin (HE) staining. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining were used to detect the expression of CCR6mRNA and its protein in rat airway. Results 1 the total number of leukocytes, eosinophils, neutrophils in BALF in asthma group was higher than that in control group and smoke exposure group (P 0.05), and the total number of leukocyte and neutrophil in asthma smoke exposure group was higher than that in asthma group. Eosinophils were lower than asthma group (P 0.05.2), and the expression of CCR6mRNA and its protein in asthma smoke exposure group were significantly higher than those in control group and smoke exposure group (P 0.01). The expression of CCR6mRNA and its protein in asthmatic smoke exposure group was significantly higher than that in asthma group (P < 0.01). Conclusion the high expression of CCR6mRNA and its protein in airway mucosa suggests that CCR6 may be involved in airway inflammation in asthmatic rats, and smoke exposure may induce and aggravate chronic airway inflammation in asthmatic rats by increasing the expression of CCR6mRNA and its protein in the airway.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R562.25
[Abstract]:Objective to study the effect of tobacco smoke exposure on the expression of CCR6 (Chemokinereceptor6) in airway of asthmatic rats, and to explore the immunological mechanism of airway inflammation induced and aggravated by smoking. Methods 40 male Wistar rats were randomly divided into control group, smoke exposure group, asthma group and asthma smoke exposure group with 10 rats in each group. The rat model of asthma and the model of tobacco smoke exposure in asthmatic rats were established. The white blood cell count and cell classification of bronchoalveolar lavage fluid (BALF) were collected and the pathological changes of lung tissue were observed by hematoxylin and eosin (HE) staining. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining were used to detect the expression of CCR6mRNA and its protein in rat airway. Results 1 the total number of leukocytes, eosinophils, neutrophils in BALF in asthma group was higher than that in control group and smoke exposure group (P 0.05), and the total number of leukocyte and neutrophil in asthma smoke exposure group was higher than that in asthma group. Eosinophils were lower than asthma group (P 0.05.2), and the expression of CCR6mRNA and its protein in asthma smoke exposure group were significantly higher than those in control group and smoke exposure group (P 0.01). The expression of CCR6mRNA and its protein in asthmatic smoke exposure group was significantly higher than that in asthma group (P < 0.01). Conclusion the high expression of CCR6mRNA and its protein in airway mucosa suggests that CCR6 may be involved in airway inflammation in asthmatic rats, and smoke exposure may induce and aggravate chronic airway inflammation in asthmatic rats by increasing the expression of CCR6mRNA and its protein in the airway.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R562.25
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