高氧促进不成熟肺组织HO-1表达的研究

发布时间：2018-07-26 19:39

【摘要】：目的研究持续高氧暴露时,新生大鼠不成熟肺组织中血红素加氧酶-1(HO-1)的表达,推测其在高氧肺损伤机理中的作用。方法随机将足月SD新生大鼠80只分为空气组和高氧组,每组40只。高氧组出生后立即置入氧体积分数95%的持续高氧环境中饲养,空气组则持续空气中饲养。两组分别于暴露于高氧或空气中4、7、10d时随机抽取8只,麻醉后取肺组织,比较两组肺组织的病理学改变,采用免疫组化法检测HO-1在肺组织中的表达,并计算两组肺组织面积积分光密度(IOD)值。结果病理学检查显示,高氧7d,可见肺泡结构破坏,肺泡壁大量炎细胞侵润,肺间隔增宽,可见明显肺水肿改变。高氧10d,肺体积缩小,正常肺泡数目减少。肺泡结构破坏,局部纤维增生,肺纤维化明显。肺组织中血红素加氧酶-1蛋白阳性表达主要表现为肺泡上皮细胞、支气管上皮细胞及肺泡巨噬细胞胞浆呈棕黄色,高氧4、7、10d组IOD值表明各时间点肺组织的HO-1表达均高于空气组(P0.05)。结论持续高浓度氧可促进不成熟肺组织HO-1表达增加,这种表达增加可能与不成熟肺组织高氧肺损伤病理生理有关。
[Abstract]:Objective to investigate the expression of heme oxygenase-1 (HO-1) in immature lung tissues of neonatal rats exposed to hyperoxia and to speculate its role in the mechanism of hyperoxia lung injury. Methods 80 adult Sprague-Dawley rats were randomly divided into air group and hyperoxia group with 40 rats in each group. The hyperoxia group was fed with 95% oxygen volume fraction immediately after birth, while the air group was kept in the air. Eight lung tissues were randomly selected after 10 days of exposure to hyperoxia or air. The pathological changes of lung tissue were compared between the two groups. The expression of HO-1 in lung tissue was detected by immunohistochemical method. The integral optical density (IOD) of lung tissue area in both groups was calculated. Results pathological examination showed that at 7 days of hyperoxia, the alveolar structure was destroyed, a large number of inflammatory cells in the alveolar wall invaded, the pulmonary septum widened, and obvious changes of pulmonary edema were observed. After 10 days of hyperoxia, the volume of lung decreased and the number of normal alveoli decreased. The alveolar structure was destroyed, local fibrous hyperplasia and pulmonary fibrosis were observed. The positive expression of heme oxygenase-1 protein in lung tissues was mainly found in alveolar epithelial cells, and the cytoplasm of bronchial epithelial cells and alveolar macrophages was brown. The IOD value of hyperoxia group for 10 days showed that the expression of HO-1 in lung tissue was higher than that in air group at each time point (P0.05). Conclusion continuous high oxygen concentration can promote the increase of HO-1 expression in immature lung tissues, which may be related to the pathophysiology of hyperoxia lung injury in immature lung tissues.
【作者单位】：四川大学华西第二医院麻醉科;
【基金】：四川省科技厅支撑计划项目资助(No.2011SZ0200)资助
【分类号】：R563.8

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